Momchilova-Pankova A B, Markovska T T, Koshlukova S E, Koumanov K S
Central Laboratory of Biophysics, Bulgarian Academy of Sciences, Sofia.
J Lipid Mediat. 1991 Mar-Apr;3(2):215-23.
Investigations have been carried out on the lipid dependence of membrane-bound phosphatidylinositol-specific phospholipase C in rat liver plasma membranes. For this purpose the phospholipid composition of rat liver plasma membranes has been modified in two different ways. The first method included enrichment of plasma membranes with different phospholipids in the presence of lipid transfer proteins, and the second a partial delipidation by means of exogenous phospholipases A2 and C and selective enrichment with different phospholipids. The results indicated that almost all used phospholipids induced activation of phosphatidylinositol-specific phospholipase C except sphingomyelin. Phosphatidylethanolamine and egg yolk phosphatidylcholine were observed to be most effective in phospholipase C activation.
已对大鼠肝细胞膜中膜结合的磷脂酰肌醇特异性磷脂酶C的脂质依赖性进行了研究。为此，大鼠肝细胞膜的磷脂组成已通过两种不同方式进行了改变。第一种方法包括在脂质转移蛋白存在的情况下用不同的磷脂富集细胞膜，第二种方法是通过外源性磷脂酶A2和C进行部分脱脂，并选择性地用不同的磷脂富集。结果表明，除鞘磷脂外，几乎所有使用的磷脂都能诱导磷脂酰肌醇特异性磷脂酶C的激活。观察到磷脂酰乙醇胺和蛋黄磷脂酰胆碱在激活磷脂酶C方面最有效。
