5'-核苷酸酶和磷脂酶A2对肝细胞膜修饰的敏感性。

Sensitivity of 5'-nucleotidase and phospholipase A2 towards liver plasma membranes modifications.

作者信息

Momchilova A, Petkova D, Mechev I, Dimitrov G, Koumanov K

出版信息

Int J Biochem. 1985;17(7):787-92. doi: 10.1016/0020-711x(85)90265-4.

Abstract

The changes in the phospholipid and fatty acid composition of liver plasma membranes isolated from rats, fed two different diets, containing either saturated or unsaturated fatty acids, were investigated. We established that dietary treatment can considerably modify the fatty acid as well as the phospholipid composition of liver plasma membranes. Lipid transfer proteins were used for enrichment of liver plasma membranes with sphingomyelin, dioleoylphosphatidylcholine, dipalmitoylphosphatidylcholine, and phosphatidylinositol. A marked sphingomyelin and membrane fluidity dependence of the membrane-bound 5'-nucleotidase and phospholipase A2 was observed.

摘要

研究了从喂食两种不同饮食（分别含有饱和脂肪酸或不饱和脂肪酸）的大鼠中分离出的肝细胞膜磷脂和脂肪酸组成的变化。我们确定饮食处理可显著改变肝细胞膜的脂肪酸以及磷脂组成。使用脂质转移蛋白用鞘磷脂、二油酰磷脂酰胆碱、二棕榈酰磷脂酰胆碱和磷脂酰肌醇富集肝细胞膜。观察到膜结合的5'-核苷酸酶和磷脂酶A2对鞘磷脂和膜流动性有显著依赖性。