[多瘤病毒负荷量与出血性膀胱炎之间的相关性]
[Correlation between load of polyomavirus and hemorrhagic cystitis].
作者信息
Tong Chun-Rong, Teng Zhi-Ping, Liu Hong-Xing, Cai Peng, Ma Si-Kun, Zhen Cheng-Liang, Zeng Yi, Lu Dao-Pei
机构信息
Beijing Dao-Pei Hospital, Beijing, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Sep;21(3):244-6.
OBJECTIVE
To study the correlation between polyoma virus load and hemorrhagic cystitis after allogeneic stem cells transplantation for prevention of hemorrhagic cystitis.
METHODS
Blood and urine specimens were collected from 40 healthy persons, 40 patient with stem cells transplantation and 20 cases complicated with hemorrhagic cystitis for determination of VP1 gene of polyomaviruses BK virus (BKV)/Jamestown Canyon virus (JCV) and simian virus 40 (SV40) by polymerase chain reaction (PCR) and EvaGreen stain fluorescence quantitative assay.
RESULTS
In the peripheral blood, all genes of BKV/JCV and SV40 were negative, while BKV gene in urine and blood from healthy persons and patient with stem cells transplantation was 15% (6/40) and 100% (40/40), respectively. The gene of JCV was positive in 10% (4/40) and 12% (5/40), the gene of SV40 was negative.
CONCLUSION
Genes of BKV and JCV was detectable in urine specimens of healthy persons and there was a correlation between the load of polyomavirus and incidence of hemorrhagic cystitis.
目的
研究异基因干细胞移植后多瘤病毒载量与出血性膀胱炎之间的相关性，以预防出血性膀胱炎。
方法
收集40例健康人、40例干细胞移植患者及20例合并出血性膀胱炎患者的血液和尿液标本，采用聚合酶链反应（PCR）和EvaGreen染色荧光定量分析法检测多瘤病毒BK病毒（BKV）/詹姆斯敦峡谷病毒（JCV）和猿猴病毒40（SV40）的VP1基因。
结果
外周血中，BKV/JCV和SV40的所有基因均为阴性，而健康人和干细胞移植患者尿液及血液中的BKV基因分别为15%（6/40）和100%（40/40）。JCV基因阳性率分别为10%（4/40）和12%（5/40），SV40基因均为阴性。
结论
健康人尿液标本中可检测到BKV和JCV基因，多瘤病毒载量与出血性膀胱炎的发生率之间存在相关性。
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