Department of Public Health Sciences, Sapienza University, Rome, Italy.
J Med Virol. 2010 Jan;82(1):138-45. doi: 10.1002/jmv.21558.
Hemorrhagic cystitis is characterized by hematuria due to inflammation of the bladder. In bone marrow transplants, this disease is linked to the infection by human polyomavirus BK, whereas the role of the human polyomavirus JC is unclear. The transcriptional control regions of both viruses contain important cellular transcription factor binding sites that undergo rearrangement process generating suitable variants that could be more active for viral replication and for the onset of hemorrhagic cystitis. In this study urine obtained from seven patients with bone marrow transplant were examined. Polyomavirus genomes were quantified by PCR and viral loads were compared. The transcriptional regions of both viruses were amplified and sequenced to determine the presence of variants. Subtypes of polyomaviruses were determined by amplification and sequencing of the viral protein 1 region. The results showed that four of seven patients were positive for BK DNA, two of seven patients had BK and JC DNA and one of seven had JC DNA. Positive samples were amplified and sequenced successively for transcriptional regions. The viral archetype was always found in both viruses. Finally, typing showed that BK virus subtype I infected patients with BK, whereas JC virus genotype IA and genotype 1B were found in patients infected with JC. The data suggest that new and different approaches are required to improve the morbidity and mortality caused by polyoma-associated hemorrhagic cystitis, since it known that BK virus is involved in the onset of hemorrhagic cystitis, whereas the role of JC virus should be investigated further.
出血性膀胱炎的特征是由于膀胱炎症引起的血尿。在骨髓移植中，这种疾病与人类多瘤病毒 BK 的感染有关，而人类多瘤病毒 JC 的作用尚不清楚。两种病毒的转录控制区都包含重要的细胞转录因子结合位点，这些位点会发生重排过程，产生更适合病毒复制和出血性膀胱炎发病的变异体。在这项研究中，检查了来自 7 名骨髓移植患者的尿液。通过 PCR 定量测定多瘤病毒基因组，比较病毒载量。扩增和测序两种病毒的转录区，以确定是否存在变异体。通过扩增和测序病毒蛋白 1 区来确定多瘤病毒的亚型。结果显示，7 名患者中有 4 名 BK DNA 阳性，7 名患者中有 2 名 BK 和 JC DNA 阳性，7 名患者中有 1 名 JC DNA 阳性。阳性样本成功扩增和测序转录区。两种病毒总是发现病毒原型。最后，分型表明 BK 病毒亚型 I 感染了 BK 病毒患者，而 JC 病毒基因型 IA 和基因型 1B 则在感染 JC 病毒的患者中发现。数据表明，需要采取新的和不同的方法来改善多瘤相关出血性膀胱炎引起的发病率和死亡率，因为已知 BK 病毒参与出血性膀胱炎的发病，而 JC 病毒的作用需要进一步研究。
