Li Yousheng, Bao Yang, Jiang Tao, Tan Li, Liu Fangnan, Li Jieshou
Department of Surgery, Jinling Hospital, Nanjing University School of Medicine, Nanjing, China.
J Invest Surg. 2007 Sep-Oct;20(5):301-6. doi: 10.1080/08941930701598826.
This study was devised to investigate whether fibrin glue (FG) in combination with growth hormone (GH) could have a beneficial effect at a late period (14 days) after injury. Male Wistar rats, with abdominal sepsis induced by an incomplete anastomosis, were divided into three groups. In the control group, the rats got incomplete anastomoses sutured alone; in the FG and FG/GH groups, anastomoses protection was performed with application of FG alone or in combination with GH. The anastomotic bursting pressure (ABP) was significantly higher in the FG/GH group than that of the FG group on postoperative day (POD) 5 (p < .01), while it could not be measured from POD 7 to POD 14 because of intestinal dehiscence. There was no difference between FG and FG/GH group on POD 3 and POD 5 in anastomotic tensile strength, which was significantly higher in the FG/GH group than that of the FG group from POD 7 to POD 14 (p < .001). Hydroxyproline content of the FG/GH group was significantly higher than that of the control from POD 3 and that of the FG group from POD 5 (p < .05). Combination of FG with GH had a synergistic effect to improve intestinal anastomotic healing over a limited 14-day course of observation.
本研究旨在探讨纤维蛋白胶(FG)联合生长激素(GH)在损伤后期(14天)是否具有有益作用。将因不完全吻合导致腹部脓毒症的雄性Wistar大鼠分为三组。对照组大鼠仅进行不完全吻合缝合;在FG组和FG/GH组中,分别单独应用FG或FG与GH联合应用进行吻合口保护。术后第5天(POD 5),FG/GH组的吻合口破裂压力(ABP)显著高于FG组(p <.01),而从POD 7至POD 14由于肠裂开无法测量。在POD 3和POD 5时,FG组和FG/GH组的吻合口抗张强度无差异,从POD 7至POD 14,FG/GH组的吻合口抗张强度显著高于FG组(p <.001)。从POD 3起,FG/GH组的羟脯氨酸含量显著高于对照组,从POD 5起显著高于FG组(p <.05)。在为期14天的有限观察过程中,FG与GH联合应用对改善肠吻合口愈合具有协同作用。