Yoshida Yasuko, Ito Azusa, Murakami Masashi, Murakami Takayuki, Fujimoto Hideharu, Takeda Kikuo, Suzuki Shigeru, Hori Masahiro
Sumika Chemical Analysis Service, Ltd., Chiba, Japan.
J Air Waste Manag Assoc. 2007 Oct;57(10):1164-71. doi: 10.3155/1047-3289.57.10.1164.
Alkylphenol polyethoxylates (APEOs) have been widely used as nonionic surfactants in a variety of industrial and commercial products. Typical compounds are nonylphenol polyethoxylates (NPEOs) and octylphenol polyethoxylates (OPEOs), which serve as precursors to nonylphenol (NP) and octylphenol (OP), respectively. NP and 4-t-OP are known to have endocrine disrupting effects on fish (medaka, Oryzias latipes), so it is important to know the concentrations of APEOs in the environment. Because the analytical characteristics of these compounds depend on the length of the ethoxy chain, it is necessary to use appropriate compounds as internal standards or surrogates. We synthesized two 13C-labeled surrogate compounds and used these compounds as internal standards to determine NPEOs and OPEOs by high-performance liquid chromatography (LC)-mass spectrometry. Method detection limits were 0.015 microg/L for NP (2)EO to 0.037 microg/L for NP(12)EO, and 0.011 microg/L for OP(3,6)EO to 0.024 microg/L for OP (4)EO. NPEO concentrations in water from a sewage treatment plant were less than 0.05-0.52 microg/L for final effluent and 1.2-15 microg/L for influent. OPEO concentrations were less than 0.05-0.15 microg/L for the final effluent and less than 0.05-1.1 microg/L for influent.
烷基酚聚乙氧基化物（APEOs）作为非离子表面活性剂已被广泛应用于各种工业和商业产品中。典型的化合物是壬基酚聚乙氧基化物（NPEOs）和辛基酚聚乙氧基化物（OPEOs），它们分别是壬基酚（NP）和辛基酚（OP）的前体。已知NP和4 -叔辛基酚（4-t-OP）对鱼类（青鳉，Oryzias latipes）具有内分泌干扰作用，因此了解环境中APEOs的浓度很重要。由于这些化合物的分析特性取决于乙氧基链的长度，所以有必要使用合适的化合物作为内标或替代物。我们合成了两种13C标记的替代化合物，并将这些化合物用作内标，通过高效液相色谱（LC）-质谱法测定NPEOs和OPEOs。方法检测限为NP（2）EO的0.015微克/升至NP（12）EO的0.037微克/升，以及OP（3,6）EO的0.011微克/升至OP（4）EO的0.024微克/升。污水处理厂出水中NPEO的浓度，最终出水小于0.05 - 0.52微克/升，进水为1.2 - 15微克/升。OPEO的浓度，最终出水小于0.05 - 0.15微克/升，进水小于0.05 - 1.1微克/升。
