He Cheng-Qiang, Ding Nai-Zheng, Fan Wei
College of Life Science, Shandong Normal University, Shandong Province 250014, China.
Mol Cell Biochem. 2008 Jan;308(1-2):247-52. doi: 10.1007/s11010-007-9632-1. Epub 2007 Nov 1.
Peroxisome proliferator-activated receptors delta (PPARdelta) is a nuclear hormone receptor belonging to the steroid receptor superfamily and is molecular targets for drugs to treat hypertriglyceridemia and type 2 diabetes. Yin Yang 1 (YY1) is a transcription factor that can repress or activate transcription of the genes with which it interacts. In this report, we show that YY1 specifically interacts with the PPARdelta promoter. Overexpression of YY1 in Hela and NIH 3T3 cells repressed the activity of the PPARdelta promoter, while the PPARdelta promoter activity was enhanced when YY1 was knocked down by siRNA YY1. We also show that YY1 in nuclear extracts was able to bind the PPARdelta promoter directly. These results suggest that YY1 might be a negative regulator of PPARdelta gene expression through its direct interaction with the PPARdelta promoter.
过氧化物酶体增殖物激活受体δ（PPARδ）是属于类固醇受体超家族的核激素受体，是治疗高甘油三酯血症和2型糖尿病药物的分子靶点。阴阳1（YY1）是一种转录因子，可抑制或激活与其相互作用基因的转录。在本报告中，我们表明YY1与PPARδ启动子特异性相互作用。在Hela和NIH 3T3细胞中过表达YY1可抑制PPARδ启动子的活性，而当用siRNA YY1敲低YY1时，PPARδ启动子活性增强。我们还表明，核提取物中的YY1能够直接结合PPARδ启动子。这些结果表明，YY1可能通过与PPARδ启动子的直接相互作用成为PPARδ基因表达的负调节因子。
