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Novel mass tags for single nucleotide polymorphism detection.

作者信息

Birikh Klara R, Korshun Vladimir A, Bernad Pablo L, Malakhov Andrei D, Milner Natalie, Khan Safraz, Southern Edwin M, Shchepinov Mikhail S

机构信息

Shemyakin-Ovchinikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya 16/10, 117997, Moscow, Russia.

出版信息

Anal Chem. 2008 Apr 1;80(7):2342-50. doi: 10.1021/ac071291y. Epub 2007 Oct 31.

DOI:10.1021/ac071291y
PMID:17973350
Abstract

A new method suitable for single nucleotide polymorphism detection and other applications based on oligonucleotide probe extension has been developed. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption/ionization of the cleavable labels. A new family of sulfur-linked laser-cleavable trityl labels with vastly improved flying abilities is implemented in this study. Corresponding reagents compatible with automated oligonucleotide synthesis are presented. Utility of this method for SNP genotyping is demonstrated.

摘要

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