Birikh Klara R, Bernad Pablo L, Shmanai Vadim V, Malakhov Andrei D, Shchepinov Mikhail S, Korshun Vladimir A
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya, Moscow, Russia.
Methods Mol Biol. 2009;578:345-61. doi: 10.1007/978-1-60327-411-1_21.
A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.
一种适用于使用差异寡核苷酸探针延伸进行单核苷酸多态性（SNP）检测的新方法已被开发出来。该方案采用了硫连接的可激光切割的三苯甲基标签。该方法基于质谱分析，并利用单一表面对延伸探针进行亲和纯化以及对可切割标签进行无基质解吸电离。证明了该方法在SNP基因分型中的实用性。
