使用三苯甲基质量标签进行单核苷酸多态性检测。

SNP detection using trityl mass tags.

作者信息

Birikh Klara R, Bernad Pablo L, Shmanai Vadim V, Malakhov Andrei D, Shchepinov Mikhail S, Korshun Vladimir A

机构信息

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya, Moscow, Russia.

出版信息

Methods Mol Biol. 2009;578:345-61. doi: 10.1007/978-1-60327-411-1_21.

DOI:10.1007/978-1-60327-411-1_21

PMID:19768604

Abstract

A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.

摘要

一种适用于使用差异寡核苷酸探针延伸进行单核苷酸多态性（SNP）检测的新方法已被开发出来。该方案采用了硫连接的可激光切割的三苯甲基标签。该方法基于质谱分析，并利用单一表面对延伸探针进行亲和纯化以及对可切割标签进行无基质解吸电离。证明了该方法在SNP基因分型中的实用性。