聚乙二醇化脂质体复合物：影响DNA凝聚和细胞转染效率的制备方案

PEGylated lipoplexes: preparation protocols affecting DNA condensation and cell transfection efficiency.

作者信息

Bombelli Cecilia, Faggioli Francesca, Luciani Paola, Mancini Giovanna, Sacco Maria Grazia

机构信息

Istituto di Metodologie Chimiche del CNR and Dipartimento di Chimica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, 00185 Rome, Italy.

出版信息

J Med Chem. 2007 Nov 29;50(24):6274-8. doi: 10.1021/jm061260f. Epub 2007 Oct 31.

DOI:10.1021/jm061260f

PMID:17973357

Abstract

The inclusion of poly(ethylene glycol) monolaurate in liposomes formulated with dimyristoyl-sn-glycero-3-phosphocholine and certain cationic gemini surfactants improves their capability of condensing DNA into a psi phase and transfecting it into cells. Both the condensation, observed by circular dichroism, and the transfection efficiency are strongly effected by the protocol of inclusion of the polymer in the formulations. The highest transfection efficiency is observed in correspondence of the highest extent of DNA condensation.

摘要

在由二肉豆蔻酰-sn-甘油-3-磷酸胆碱和某些阳离子双子表面活性剂配制的脂质体中加入聚乙二醇单月桂酸酯，可提高它们将DNA浓缩成ψ相并将其转染到细胞中的能力。通过圆二色性观察到的凝聚以及转染效率都受到聚合物在制剂中加入方案的强烈影响。在DNA凝聚程度最高时观察到最高的转染效率。