Stanić Vesna, Arntz Youri, Richard Doriane, Affolter Christine, Nguyen Isabelle, Crucifix Corinne, Schultz Patrick, Baehr Corinne, Frisch Benoit, Ogier Joelle
INSERM UMR 595, Institut National de la Sante et de la Recherche Medicale, UFR d'Odontologie, Universite Louis Pasteur, F-67085 Strasbourg, France.
Biomacromolecules. 2008 Jul;9(7):2048-55. doi: 10.1021/bm800287z. Epub 2008 Jun 24.
In this paper we propose a detailed analysis of structural and morphological properties of two poly-L-lysine (PLL)-based transfection formulations, PLL/DNA and pegylated PLL (PLL-g-PEG)/DNA, by means of atomic force microscopy (AFM) and transmission electron microscopy (TEM). Comparing PLL-g-PEG/DNA with PLL/DNA polyplexes, we demonstrate that, due to the presence of PEG, the particles differ not only in size, shape, and crystalline structure, but also in transfection efficiency. While PLL condensates DNA in large agglomerates, PLL grafted with polyethylene glycol 2000 can condensate DNA in long filaments with diameters of some nanometers (6-20 nm). These structures are dependent on the grafting ratio and are more efficient than compacted ones, showing that DNA uptake and processing by cell is directly related to physicochemical properties of the polyplexes.
在本文中，我们借助原子力显微镜（AFM）和透射电子显微镜（TEM），对两种基于聚-L-赖氨酸（PLL）的转染制剂，即PLL/DNA和聚乙二醇化PLL（PLL-g-PEG）/DNA的结构和形态特性进行了详细分析。通过将PLL-g-PEG/DNA与PLL/DNA多聚体进行比较，我们证明，由于PEG的存在，颗粒不仅在大小、形状和晶体结构上有所不同，而且在转染效率上也存在差异。虽然PLL将DNA凝聚成大的团聚体，但接枝了聚乙二醇2000的PLL能将DNA凝聚成直径为几纳米（6 - 20纳米）的长丝。这些结构取决于接枝率，并且比紧密结构更有效，这表明细胞对DNA的摄取和处理与多聚体的物理化学性质直接相关。
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