Ortiz-Olvera Nayeli N X, Morán Villota S, Gallardo Wong I, Blancas Valencia J M, Cabrera Muñoz L
Universidad Autónoma del Estado de México, Mexico.
Rev Esp Enferm Dig. 2007 Jul;99(7):392-7. doi: 10.4321/s1130-01082007000700005.
To validate a simplified 13C-urea breath test (13C-UBT) method for the diagnosis of H. pylori infection.
Patients referred for gastrointestinal endoscopy and biopsy were included, and a 13C-UBT was performed after a 6-hour fast. Breath samples were collected in 10 ml glass tubes before and 30 min after the simultaneous administration of 50 mg of 13C-urea and 2 g of citric acid in 200 ml of water. All breath samples were analyzed using isotope ratio mass spectrometry. The diagnosis of H. pylori infection was established with a positive culture and/or positive histology and serology.
Eighty-eight patients were included, 49 female and 39 male with a mean age of 45 +/- 15 yrs. Fifty-one patients (57.95%) were positive and 30 (34.1%) negative for H. pylori. Seven cases (7.95%) were considered undetermined. The sensitivity, specificity, positive predictive value, and negative predictive value for 13C-UBT were 90.2, 93.3, 95.83, and 84.8%, respectively. Accuracy was 91.4%.
The simultaneous administration of 50 mg of 13C-urea and 2 g of citric acid represents an alternative for the non-invasive diagnosis of H. pylori infection.
验证一种简化的¹³C尿素呼气试验（¹³C-UBT）方法用于诊断幽门螺杆菌感染。
纳入因胃肠内镜检查和活检而转诊的患者，禁食6小时后进行¹³C-UBT。在将50mg¹³C尿素和2g柠檬酸同时溶于200ml水中后，于给药前及给药后30分钟在10ml玻璃管中采集呼气样本。所有呼气样本均采用同位素比率质谱分析法进行分析。通过阳性培养和/或阳性组织学及血清学结果来确诊幽门螺杆菌感染。
共纳入88例患者，其中49例女性，39例男性，平均年龄45±15岁。51例患者（57.95%）幽门螺杆菌检测呈阳性，30例（34.1%）呈阴性。7例（7.95%）结果不确定。¹³C-UBT的敏感性、特异性、阳性预测值和阴性预测值分别为90.2%、93.3%、95.83%和84.8%。准确率为91.4%。
同时给予50mg¹³C尿素和2g柠檬酸是幽门螺杆菌感染非侵入性诊断的一种替代方法。
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