Peng N J, Hsu P I, Lee S C, Tseng H H, Huang W K, Tsay D G, Ger L P, Lo G H, Lin C K, Tsai C C, Lai K H
Department of Nuclear Medicine, Veterans General Hospital-Kaohsiung, Taiwan, Republic of China.
J Gastroenterol Hepatol. 2000 Mar;15(3):284-9. doi: 10.1046/j.1440-1746.2000.02159.x.
Non-ulcer dyspepsia (NUD) accounts for the majority of dyspeptic patients and studies on the epidemiology of Helicobacter pylori infection in NUD depend on a non-invasive and rapid diagnostic test. This study was performed to determine the sensitivity and specificity of a 15-min simplified protocol of the [13C]-urea breath test ([13C]-UBT) for the diagnosis of H. pylori infection in patients with NUD.
One hundred and thirty-six patients with a clinical and endoscopic diagnosis of NUD were included. The [13C]-UBT was modified from the European standard protocol. The baseline breath sample was collected 5 min after the patient took a test meal and the 13CO2 was collected 15 min after the patient drank 100 mg [13C]-urea. The gold standard used for comparison was either a positive culture or positive histology + positive rapid urease test sampled on upper gastrointestinal endoscopy.
The prevalence of H. pylori infection in NUD by the gold standard was 59.6%, whereas that calculated by the [13C]-UBT was 60.3%. The sensitivity and specificity of [13C]-UBT was 93.8 and 89.1% compared with the gold standard. The shortened collection time and simplification of the procedure may have led to a decline in specificity.
The 15-min [13C]-UBT is a rapid but less specific protocol for detecting the presence of H. pylori infection in patients with NUD.
非溃疡性消化不良（NUD）占消化不良患者的大多数，关于NUD患者幽门螺杆菌感染流行病学的研究依赖于一种非侵入性快速诊断测试。本研究旨在确定[¹³C] - 尿素呼气试验（[¹³C] - UBT）15分钟简化方案对NUD患者幽门螺杆菌感染诊断的敏感性和特异性。
纳入136例经临床和内镜诊断为NUD的患者。[¹³C] - UBT由欧洲标准方案修改而来。在患者进食试验餐5分钟后采集基线呼气样本，在患者饮用100毫克[¹³C] - 尿素15分钟后采集¹³CO₂样本。用于比较的金标准是上消化道内镜检查时阳性培养或阳性组织学 + 阳性快速尿素酶试验。
根据金标准，NUD患者中幽门螺杆菌感染率为59.6%，而通过[¹³C] - UBT计算的感染率为60.3%。与金标准相比，[¹³C] - UBT的敏感性和特异性分别为93.8%和89.1%。采集时间的缩短和程序的简化可能导致特异性下降。
15分钟[¹³C] - UBT是一种快速但特异性较低的检测NUD患者幽门螺杆菌感染的方案。
