神经肽Y放射免疫测定：特性与应用

Neuropeptide Y radio-immunoassay: characterization and application.

Allen R, Boublik J, Hauger R L, Scott N, Rivier J, Brown M R

Dept of Medicine, University of California, San Diego, La Jolla 92093-0817.

Clin Exp Pharmacol Physiol. 1991 Dec;18(12):825-33. doi: 10.1111/j.1440-1681.1991.tb01402.x.

Abstract

A sensitive and specific neuropeptide Y (NPY) radio-immunoassay has been developed. This radio-immunoassay does not detect the NPY-related peptides pancreatic polypeptide or peptide YY. NPY extracted from rat plasma using sequential C18 sorbent and affinity chromatography co-eluted with synthetic rat NPY when applied to high pressure liquid chromatography. 2. The procedure of stabilization of platelets followed by high speed centrifugation reduced basal values of NPY by 60%, and this may be consistent with removal of platelets that release NPY. Administration of the cholinesterase inhibitor physostigmine (0.3 mg/kg), intravenously, produced a small but significant increase (39%) from basal concentrations of NPY. 3. NPY concentrations in young (2-3-month-old) Sprague-Dawley and Fisher 944 rats were similar; however, NPY concentrations were significantly increased (55%) in 2-year-old Fisher 944 rats. Similar to plasma concentrations of noradrenaline, NPY levels increase with age.

摘要

已开发出一种灵敏且特异的神经肽Y（NPY）放射免疫测定法。该放射免疫测定法无法检测与NPY相关的肽类，即胰多肽或肽YY。当将用连续C18吸附剂和亲和色谱法从大鼠血浆中提取的NPY应用于高压液相色谱时，其与合成大鼠NPY共洗脱。2. 先稳定血小板然后进行高速离心的操作使NPY的基础值降低了60%，这可能与去除释放NPY的血小板有关。静脉注射胆碱酯酶抑制剂毒扁豆碱（0.3mg/kg），使NPY的基础浓度有小幅但显著的升高（39%）。3. 年轻（2至3月龄）的斯普拉格-道利大鼠和费希尔944大鼠的NPY浓度相似；然而，2岁的费希尔944大鼠的NPY浓度显著升高（55%）。与去甲肾上腺素的血浆浓度相似，NPY水平随年龄增长而升高。