Higuchi H, Yang H Y, Costa E
Department of Pharmacology I, School of Medicine, Osaka University, Japan.
J Neurochem. 1988 Jun;50(6):1879-86. doi: 10.1111/j.1471-4159.1988.tb02492.x.
Age-related changes in neuropeptide Y (NPY) regulation were studied in rat adrenal glands, brains, and blood by radioimmunoassay and biochemical characterization using reversed phase HPLC and gel filtration chromatography. NPY immunoreactivity (pmol/g tissue +/- SEM) in rat adrenal glands increased from 7 +/- 1 (6 weeks old) to 1,500 +/- 580 (69 weeks old). Biochemical characterization by HPLC showed that this increase was due to those of NPY and methionine sulfoxide NPY. In contrast, in rat brain, NPY content decreased in an age-dependent manner specifically in striatum, hippocampus, medulla oblongata, and spinal cord and the sulfoxide form was not detected. In rat blood, the circulating level of NPY was high (3-5 pmol/ml plasma +/- SEM) but did not change significantly with age or by adrenal demedullation. Only a small increase of the sulfoxide form of NPY was observed in aged rat plasma. The age-dependent changes in regulation and modification of NPY in adrenal glands and in specific brain areas may have physiological relevance in the regulation of catecholamine release from adrenal glands and some brain functions during aging.
通过放射免疫分析以及使用反相高效液相色谱法和凝胶过滤色谱法进行生化特性分析，研究了大鼠肾上腺、大脑和血液中神经肽Y（NPY）调节的年龄相关变化。大鼠肾上腺中的NPY免疫反应性（每克组织皮摩尔数±标准误）从7±1（6周龄）增加到1500±580（69周龄）。高效液相色谱法的生化特性分析表明，这种增加是由于NPY和甲硫氨酸亚砜NPY的增加所致。相比之下，在大鼠大脑中，NPY含量以年龄依赖性方式下降，特别是在纹状体、海马、延髓和脊髓中，且未检测到亚砜形式。在大鼠血液中，NPY的循环水平较高（3 - 5皮摩尔/毫升血浆±标准误），但并未随年龄或肾上腺去髓质作用而发生显著变化。在老年大鼠血浆中仅观察到NPY亚砜形式有少量增加。肾上腺和特定脑区中NPY调节和修饰的年龄依赖性变化可能在衰老过程中肾上腺儿茶酚胺释放的调节以及某些脑功能方面具有生理相关性。
