Lin Yi, Zhong Yanmin, Shen Weizai, Chen Yijing, Shi Jianbo, Di Jingfang, Zeng Shan, Saito Shigeru
Institute of Tissue Transplantation and Immunology, College of Life Science and Technology, Jinan University, Guangzhou, China.
Clin Immunol. 2008 Jan;126(1):104-17. doi: 10.1016/j.clim.2007.09.006. Epub 2007 Oct 31.
Thymic stromal lymphopoietin (TSLP)-TSLP receptor (TSLP-R) interactions activate CD11c(+) dendritic cells (DCs) and increase epithelial cell Th2-type cytokine production. We detected intracellular TSLP expression on CK7(+) trophoblast cells and TSLP-R expression on placental DCs from pregnant BALB/cxC57BL/6 and NOD/SCIDxC57BL/6 mice on gestational day 12.5. Murine recombinant TSLP activated DCs from BALB/c mice, with increased CD80 and CD83 expressions; TSLP-activated DCs induced IL-10-producing NK cell expansion. This was abrogated by anti-TSLP Ab or by culturing CD49b(+) NK cells alone. No TSLP-DC-induced IL-10(+)CD49b(+) cell expansion occurred when DCs and CD49b(+) cells were cultured separately. Although TSLP-induced DC activation occurred in NOD/SCID mice, the IL-10(+) NK cell percentage was unchanged. CK7(+) trophoblast cells may activate placental DCs via a TSLP-TSLP-R interaction and induce DC-dependent placental NK cell IL-10 production. TSLP-DC and NK cell contact appears necessary for IL-10(+)CD49b(+) cell expansion. Placental NK cells from NOD/SCIDxC57BL/6 mice appear less sensitive to TSLP-DC induction.
胸腺基质淋巴细胞生成素（TSLP）-TSLP受体（TSLP-R）相互作用可激活CD11c(+)树突状细胞（DCs），并增加上皮细胞Th2型细胞因子的产生。我们在妊娠第12.5天检测了来自妊娠BALB/cxC57BL/6和NOD/SCIDxC57BL/6小鼠的CK7(+)滋养层细胞内TSLP的表达以及胎盘DCs上TSLP-R的表达。鼠重组TSLP激活了BALB/c小鼠的DCs，导致CD80和CD83表达增加；TSLP激活的DCs诱导产生白细胞介素10（IL-10）的自然杀伤（NK）细胞扩增。抗TSLP抗体或单独培养CD49b(+)NK细胞可消除这种扩增。当DCs和CD49b(+)细胞分开培养时，未发生TSLP-DC诱导的IL-10(+)CD49b(+)细胞扩增。尽管在NOD/SCID小鼠中发生了TSLP诱导的DC激活，但IL-10(+)NK细胞百分比未改变。CK7(+)滋养层细胞可能通过TSLP-TSLP-R相互作用激活胎盘DCs，并诱导DC依赖的胎盘NK细胞产生IL-10。TSLP-DC与NK细胞的接触似乎是IL-10(+)CD49b(+)细胞扩增所必需的。来自NOD/SCIDxC57BL/6小鼠的胎盘NK细胞似乎对TSLP-DC诱导不太敏感。
