Silipo Alba, De Castro Cristina, Lanzetta Rosa, Molinaro Antonio, Parrilli Michelangelo, Vago Giorgio, Sturiale Luisa, Messina Angela, Garozzo Domenico
Dipartimento di Chimica Organica e Biochimica, Universitá di Napoli Federico II, via Cynthia 4, 80126 Napoli, Italy.
J Mass Spectrom. 2008 Apr;43(4):478-84. doi: 10.1002/jms.1333.
Here, a new 'one pot' and fast approach is described, based on electrospray ionization (ESI) of negative ions by using a hybrid linear ion trap/orbitrap mass spectrometer (LTQ/orbitrap) for MS and MS/MS analysis. By this method the distribution of the primary and secondary acyl residues of the intact lipid A is inferred by analysis of the ESI spectra measured in positive and negative mode. The analysis of these data allows an unequivocal assignment of the fatty acid distribution. This methodology was successfully tested on two different lipid A with known structures, deriving from the Agrobacterium tumefaciens and Escherichia coli lipopolysaccharides (LPS).
本文描述了一种基于负离子电喷雾电离（ESI）的新型“一锅法”快速方法，该方法使用混合线性离子阱/轨道阱质谱仪（LTQ/轨道阱）进行质谱（MS）和串联质谱（MS/MS）分析。通过这种方法，通过分析正负模式下测得的ESI光谱，可以推断出完整脂质A的一级和二级酰基残基的分布。对这些数据的分析能够明确脂肪酸的分布。该方法已成功应用于两种结构已知的不同脂质A，它们分别来源于根癌土壤杆菌和大肠杆菌的脂多糖（LPS）。
