Castro Joao V, Peralba Maria C R, Ayub Marco A Z
Institute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.
J Environ Sci Health B. 2007 Nov;42(8):883-6. doi: 10.1080/03601230701623290.
The biodegradation conducted by microorganisms on herbicide glyphosate (N-phosphonomethylglycine) was investigated. Five strains of filamentous fungi belonging to the Fusarium genre were grown on Czapeck medium without phosphorous and supplemented with the addition of glyphosate. The assays were conducted to determine the ability of use as a phosphorous source, the inhibition caused by presence of herbicide, and the biodegradation in shaker and bioreactor by Fusarium strains. It was observed that the herbicide did not show any negative effect on microrganisms by quantity of the biomass. Among the strains tested, no inhibition was noted by the addition of glyphosate even at a high concentration. All strains studied were able to biodegrade it and use the herbicide as a phosphorous source. The formation of consortium was not better than the strains tested in pure culture. The biodegradation in the bioreactor was better than in the shaker. However, there wasn't any influence on biodegradation rate by changing the amount of oxygen in the system.
对微生物对除草剂草甘膦（N-膦酰基甲基甘氨酸）的生物降解进行了研究。将属于镰刀菌属的五株丝状真菌在不含磷并添加了草甘膦的查氏培养基上培养。进行了测定以确定用作磷源的能力、除草剂存在引起的抑制作用以及镰刀菌菌株在摇床和生物反应器中的生物降解情况。观察到除草剂对生物量数量的微生物没有显示出任何负面影响。在所测试的菌株中，即使添加高浓度的草甘膦也未观察到抑制作用。所有研究的菌株都能够对其进行生物降解并将除草剂用作磷源。形成的菌群并不比纯培养中测试的菌株更好。在生物反应器中的生物降解比在摇床中更好。然而，通过改变系统中的氧气量对生物降解速率没有任何影响。
