Lee You Jin, Park Do Joon, Shin Chan Soo, Park Kyong Soo, Kim Seong Yeon, Lee Hong Kyu, Park Young Joo, Cho Bo Youn
Department of Internal Medicine, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.
J Korean Med Sci. 2007 Oct;22(5):883-90. doi: 10.3346/jkms.2007.22.5.883.
To determine which genes are regulated by thyroid stimulating hormone (thyrotropin, TSH), insulin and insulin-like growth factor-1 (IGF-1) in the rat thyroid, we used the microarray technology and observed the changes in gene expression. The expressions of genes for bone morphogenetic protein 6, the glucagon receptor, and cyclin D1 were increased by both TSH and IGF-1; for cytochrome P450, 2c37, the expression was decreased by both. Genes for cholecystokinin, glucuronidase, beta, demethyl-Q 7, and cytochrome c oxidase, subunit VIIIa, were up-regulated; the genes for ribosomal protein L37 and ribosomal protein L4 were down-regulated by TSH and insulin. However, there was no gene observed to be regulated by all three: TSH, IGF-1, and insulin molecules studied. These findings suggest that TSH, IGF-1, and insulin stimulate different signal pathways, which can interact with one another to regulate the proliferation of thyrocytes, and thereby provide additional influence on the process of cellular proliferation.
为了确定在大鼠甲状腺中哪些基因受促甲状腺激素（促甲状腺素，TSH）、胰岛素和胰岛素样生长因子-1（IGF-1）调控，我们使用了微阵列技术并观察基因表达的变化。骨形态发生蛋白6、胰高血糖素受体和细胞周期蛋白D1的基因表达在TSH和IGF-1作用下均增加；细胞色素P450 2c37的表达在二者作用下均降低。胆囊收缩素、β-葡萄糖醛酸酶、去甲基-Q 7和细胞色素c氧化酶亚基VIIIa的基因上调；核糖体蛋白L37和核糖体蛋白L4的基因在TSH和胰岛素作用下下调。然而，未观察到有基因受所研究的TSH、IGF-1和胰岛素这三种分子共同调控。这些发现表明，TSH、IGF-1和胰岛素刺激不同的信号通路，这些信号通路可相互作用以调节甲状腺细胞的增殖，从而对细胞增殖过程产生额外影响。
