Constantinescu R, Constantinescu A T, Reichmann H, Janetzky B
Department of Neurology, Faculty of Medicine Carl Gustav Carus, Dresden University of Technology, Dresden, Germany.
J Neural Transm Suppl. 2007(72):17-28. doi: 10.1007/978-3-211-73574-9_3.
Parkinson's disease (PD) is the second most prevalent neurodegenerative disorder in industrialized countries. Present cell culture models for PD rely on either primary cells or immortal cell lines, neither of which allow for long-term experiments on a constant population, a crucial requisite for a realistic model of slowly progressing neurodegenerative diseases. We differentiated SH-SY5Y human dopaminergic neuroblastoma cells to a neuronal-like state in a perfusion culture system using a combination of retinoic acid and mitotic inhibitors. The cells could be cultivated for two months without the need for passage. We show, by various means, that the differentiated cells exhibit, at the molecular level, many neuronal properties not characteristic to the starting line. This approach opens the possibility to develop chronic models, in which the effect of perturbations and putative counteracting strategies can be monitored over long periods of time in a quasi-stable cell population.
帕金森病（PD）是工业化国家中第二常见的神经退行性疾病。目前用于帕金森病的细胞培养模型依赖于原代细胞或永生化细胞系，这两种细胞都无法在恒定群体上进行长期实验，而这对于缓慢进展的神经退行性疾病的真实模型来说是至关重要的条件。我们使用视黄酸和有丝分裂抑制剂的组合，在灌注培养系统中将SH-SY5Y人多巴胺能神经母细胞瘤细胞分化为神经元样状态。这些细胞可以培养两个月而无需传代。我们通过各种方法表明，分化后的细胞在分子水平上表现出许多起始细胞所没有的神经元特性。这种方法为开发慢性模型开辟了可能性，在该模型中，可以在准稳定的细胞群体中长时间监测扰动和假定的对抗策略的效果。
