Xie Danni, Deng Ting, Zhai Zhenwei, Sun Tao, Xu Ying
State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
School of Medical Information Engineering, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Front Mol Neurosci. 2023 Jan 4;15:1016559. doi: 10.3389/fnmol.2022.1016559. eCollection 2022.
Alzheimer's disease (AD) is a common age-related neurodegenerative disease characterized by progressive cognitive decline and irreversible memory impairment. Currently, several studies have failed to fully elucidate AD's cellular and molecular mechanisms. For this purpose, research on related cellular models may propose potential predictive models for the drug development of AD. Therefore, many cells characterized by neuronal properties are widely used to mimic the pathological process of AD, such as PC12, SH-SY5Y, and N2a, especially the PC12 pheochromocytoma cell line. Thus, this review covers the most systematic essay that used PC12 cells to study AD. We depict the cellular source, culture condition, differentiation methods, transfection methods, drugs inducing AD, general approaches (evaluation methods and metrics), and cellular models used in parallel with PC12 cells.
阿尔茨海默病（AD）是一种常见的与年龄相关的神经退行性疾病，其特征为进行性认知衰退和不可逆的记忆损害。目前，多项研究未能充分阐明AD的细胞和分子机制。为此，相关细胞模型的研究可能为AD药物研发提供潜在的预测模型。因此，许多具有神经元特性的细胞被广泛用于模拟AD的病理过程，如PC12细胞、SH-SY5Y细胞和N2a细胞，尤其是PC12嗜铬细胞瘤细胞系。因此，本综述涵盖了使用PC12细胞研究AD的最系统的论文。我们描述了细胞来源、培养条件、分化方法、转染方法、诱导AD的药物、一般方法（评估方法和指标）以及与PC12细胞并行使用的细胞模型。
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