补阳还五汤对神经上皮干细胞轴突生长和分化的影响。
Effects of Buyang Huanwu Decoction on neurite outgrowth and differentiation of neuroepithelial stem cells.
作者信息
Sun Jin-Hao, Gao Ying-Mao, Yang Lin, Wang Xian, Bao Li-Hua, Liu Wen-Jing, Yew David
机构信息
Institute of Anatomy and Histology & Embryology, School of Medicine, Shandong University, Jinan 250012, Shandong, P.R. China.
出版信息
Chin J Physiol. 2007 Aug 31;50(4):151-6.
To determine the effects of Buyang Huanwu Decoction (BYHWD), a traditional Chinese medicine, on neurite outgrowth and differentiation of neuroepithelial stem cells (NEPs), NEPs were isolated from embryonic neural tube and cultured in medium with rat serum containing BYHWD, which was prepared from rats administrated orally with BYHWD. The average neurite length of NEPs grew significantly longer in rat serum containing BYHWD than in control serum without BYHWD. More neurofilament (NF) positive cells and glial fibrillary acidic protein (GFAP) positive cells were detected in NEPs cultured in the presence of BYHWD. Besides, when cultured NEPs were loaded with Fluo-3-AM, the fluorescence intensity obtained from NEPs cultured in serum with BYHWD was significantly lower than that from NEPs cultured in control serum without BYHWD. Our results indicate that BYHWD could exert a promotion effect on neurite outgrowth and differentiation of NEPs.
为确定中药补阳还五汤（BYHWD）对神经上皮干细胞（NEPs）轴突生长和分化的影响，从胚胎神经管中分离出NEPs，并在含有经口服给予BYHWD的大鼠血清制备的含BYHWD的培养基中培养。与不含BYHWD的对照血清相比，含BYHWD的大鼠血清中NEPs的平均轴突长度显著更长。在BYHWD存在下培养的NEPs中检测到更多的神经丝（NF）阳性细胞和胶质纤维酸性蛋白（GFAP）阳性细胞。此外，当培养的NEPs加载Fluo-3-AM时，在含BYHWD的血清中培养的NEPs获得的荧光强度显著低于在不含BYHWD的对照血清中培养的NEPs。我们的结果表明，BYHWD可对NEPs的轴突生长和分化发挥促进作用。
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