Key Laboratory of the Ministry of Education for Experimental Teratology and Department of Anatomy, PR China.
J Ethnopharmacol. 2011 Oct 11;137(3):1095-101. doi: 10.1016/j.jep.2011.07.014. Epub 2011 Jul 18.
Buyang Huanwu decoction (BYHWD) is a traditional Chinese medicine and can be used to promote peripheral nerve regeneration. However the regenerative mechanism of BYHWD remains unclear. The objective of this study was to investigate the protective mechanisms of BYHWD in Schwann cells damaged by hydrogen peroxide (H(2)O(2)).
Schwann cells which were derived from neonatal sciatic nerves of rats were used in subsequent experiments. Schwann cells were injured by various concentrations of H(2)O(2) (0.25, 0.5 and 1mM final concentration). BYHWD (600 μg/ml final concentration) was added to the medium either simultaneously or 1h later after the addition of H(2)O(2). Subsequently, methyl thiazolyl tetrazolium (MTT) assay was performed. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) levels were also examined after 12h. The expression of Caspase 3 and the concentration of intercellular Ca(2+) ([Ca(2+)]i) were also determined.
Among three concentrations of H(2)O(2), 0.5mM H(2)O(2) induced Schwann cells swelled and neuritis disappeared after 12h. In the presence of BYHWD, MTT assay showed that more cells were viable in comparison with the H(2)O(2) injury group. Moreover, the addition of BYHWD has also increased the SOD activity with decreased in MDA level. Furthermore, the concentration of [Ca(2+)]i and expression of Caspase 3 were decreased with the addition of BYHWD in culture.
Our results revealed that BYHWD protected Schwann cells from oxidative injury. The mechanism of BYHWD promoting neural regeneration possibly associated with its anti-oxidative activity.
补阳还五汤（BYHWD）是一种中药，可用于促进周围神经再生。然而，BYHWD 的再生机制尚不清楚。本研究旨在探讨 BYHWD 对过氧化氢（H₂O₂）损伤的施万细胞的保护机制。
将来自新生大鼠坐骨神经的施万细胞用于后续实验。用不同浓度的 H₂O₂（终浓度为 0.25、0.5 和 1mM）损伤施万细胞。施万细胞加入 H₂O₂后同时或 1 小时后加入 BYHWD（终浓度 600μg/ml）。随后进行甲基噻唑基四唑（MTT）测定。12 小时后还检查超氧化物歧化酶（SOD）活性和丙二醛（MDA）水平。还测定了 Caspase 3 的表达和细胞内 Ca²⁺浓度（[Ca²⁺]i）。
在三种 H₂O₂浓度中，0.5mM H₂O₂在 12 小时后诱导施万细胞肿胀，神经炎消失。在 BYHWD 的存在下，MTT 测定显示与 H₂O₂损伤组相比，更多的细胞存活。此外，加入 BYHWD 还增加了 SOD 活性，降低了 MDA 水平。此外，加入 BYHWD 还降低了 [Ca²⁺]i 浓度和 Caspase 3 的表达。
我们的结果表明，BYHWD 可保护施万细胞免受氧化损伤。BYHWD 促进神经再生的机制可能与其抗氧化活性有关。
