Li Chenxu
Institute of Brain Sciences, Department of Physiology, Medical College, Datong University, Datong 037009, Shanxi Province, China.
Neural Regen Res. 2012 Jul 25;7(21):1605-10. doi: 10.3969/j.issn.1673-5374.2012.21.001.
After bilateral carotid artery occlusion for 30 minutes and reperfusion for 2 hours, distinct pathological changes presented in the cerebral cortex and cerebellum of rats. Compared with normal rats, nerve cell membrane fluidity significantly decreased in ischemia/reperfusion rats as detected by spin-labeling electron spin resonance, consistent with order parameter S and rotational correlation time τc measurements. Brain nerve cells from rats with ischemia/reperfusion injury were cultured with 1-100 mg/mL Buyang Huanwu decoction. Results showed that Buyang Huanwu decoction gradually increased membrane fluidity dose-dependently to normal levels, and eliminated hydroxide (OH(·)) and superoxide ( O2 (·)) free radicals dose-dependently. These findings suggest that Buyang Huanwu decoction can protect against cell membrane fluidity changes in rats with ischemia/ reperfusion injury by scavenging free radicals.
双侧颈动脉闭塞30分钟并再灌注2小时后，大鼠大脑皮层和小脑出现明显的病理变化。通过自旋标记电子自旋共振检测发现，与正常大鼠相比，缺血/再灌注大鼠神经细胞膜流动性显著降低，这与序参数S和旋转相关时间τc的测量结果一致。用1-100mg/mL补阳还五汤培养缺血/再灌注损伤大鼠的脑神经细胞。结果显示，补阳还五汤能剂量依赖性地使膜流动性逐渐增加至正常水平，并剂量依赖性地清除羟基（OH(·)）和超氧阴离子（O2(·)）自由基。这些结果表明，补阳还五汤可通过清除自由基来保护缺血/再灌注损伤大鼠的细胞膜流动性变化。
