Adams Dana R, Toner Mehmet, Langer Robert
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Langmuir. 2007 Dec 18;23(26):13013-23. doi: 10.1021/la700452k. Epub 2007 Nov 6.
Anhydrobiotic preservation has the potential to allow the processing and storage of mammalian cells in a state of suspended animation at ambient conditions in trehalose glasses; however, stresses--particularly to the lipid bilayer--during desiccation and rehydration have thus far prevented the full realization of the promise of this technique. Giant gel-phase 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) and liquid-crystalline-phase 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC) vesicles provide a model cell system with which to elucidate the role of trehalose in surface-lipid bilayer interactions, as well as the part played by lipid phase. In the absence of trehalose, DSPC liposomes adsorbed to polystyrene, producing irreversible structural changes and apparent leakage of all intravesicular solute upon drying and rehydration. Addition of trehalose significantly reduced vesicle adsorption with only transient intravesicular solute leakage for the rehydrated vesicles; however, at very low moisture contents, the vesicles underwent permanent structural changes. In contrast to the results with DSPC vesicles, DLPC vesicles largely avoided adsorption and exhibited high intravesicular solute retention when dried and rehydrated even in the absence of trehalose, despite significant internal structural changes.
在环境条件下，利用海藻糖玻璃化实现哺乳动物细胞的脱水保存并使其处于假死状态进行处理和储存具有潜在可能性；然而，迄今为止，干燥和复水过程中的压力，尤其是对脂质双层的压力，阻碍了这项技术全部潜力的实现。巨型凝胶相1,2 - 二硬脂酰 - sn - 甘油 - 3 - 磷酸胆碱（DSPC）囊泡和液晶相1,2 - 二月桂酰 - sn - 甘油 - 3 - 磷酸胆碱（DLPC）囊泡提供了一个模型细胞系统，可用于阐明海藻糖在表面脂质双层相互作用中的作用以及脂质相所起的作用。在没有海藻糖的情况下，DSPC脂质体吸附到聚苯乙烯上，干燥和复水后会产生不可逆的结构变化以及所有囊泡内溶质的明显泄漏。添加海藻糖可显著减少囊泡吸附，复水后的囊泡仅出现短暂的囊泡内溶质泄漏；然而，在非常低的水分含量下，囊泡会发生永久性结构变化。与DSPC囊泡的结果相反，DLPC囊泡在很大程度上避免了吸附，并且即使在没有海藻糖的情况下，干燥和复水后仍表现出较高的囊泡内溶质保留率，尽管内部结构发生了显著变化。
