Yamamoto Kazuo, Yasukawa Fumiko, Ito Seiichiro
Frontier Science Bioscience, University of Tokyo, Kashiwa Chiba, Japan.
Methods Mol Biol. 2007;381:401-9. doi: 10.1007/978-1-59745-303-5_21.
A multiplexed glyco-bead array method for determining the sugar-binding specificities of plant lectins was described by a bead-based flow cytometric assay. Glycopeptides with N- and O-glycans were immobilized on multiplexed beads, and the specificities of several kinds of sugar chains were measured in a single reaction. This strategy is easy, rapid, reproducible, and suitable for small samples, and allows the reliable elucidation of sugar-binding properties of lectins under identical conditions.
