Yamamoto Kazuo
Laboratory of Molecular Medicine, Department of Integrated Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan,
Methods Mol Biol. 2014;1200:319-26. doi: 10.1007/978-1-4939-1292-6_27.
Multiplexed bead array is an application that allows us to quantify multiple ligands simultaneously by using flow cytometry. Glycopeptides are immobilized on multiplexed beads, and the glycan-binding specificities of several lectins are determined. This strategy is easy, rapid, and suitable for small amount of samples, and allows the reliable elucidation under the identical condition. Such a technology is useful for analyzing characteristics and functions of lectins.