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Carbohydrate-binding specificity of lectins using multiplexed glyco-bead array.

作者信息

Yamamoto Kazuo

机构信息

Laboratory of Molecular Medicine, Department of Integrated Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa, Chiba, 277-8562, Japan,

出版信息

Methods Mol Biol. 2014;1200:319-26. doi: 10.1007/978-1-4939-1292-6_27.

DOI:10.1007/978-1-4939-1292-6_27
PMID:25117246
Abstract

Multiplexed bead array is an application that allows us to quantify multiple ligands simultaneously by using flow cytometry. Glycopeptides are immobilized on multiplexed beads, and the glycan-binding specificities of several lectins are determined. This strategy is easy, rapid, and suitable for small amount of samples, and allows the reliable elucidation under the identical condition. Such a technology is useful for analyzing characteristics and functions of lectins.

摘要

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