Kitao Naoya, Yahata Takahiro, Matsumoto Takaaki, Okamatsu-Ogura Yuko, Omachi Asako, Kimura Kazuhiro, Saito Masayuki
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 2007 Oct;69(10):1065-8. doi: 10.1292/jvms.69.1065.
Uncoupling protein 1 (UCP1) is present exclusively in brown adipose tissue, and contributes to body temperature control during cold exposure. We cloned UCP1 cDNA of plateau pika (Ochotona dauurica), a small, non-hibernating, diurnal lagomorph that inhabits in relatively cold climates and at high altitudes in Mongolia and in northern China. The nucleotide sequence of pika UCP1 was highly homologous to UCP1 of other species, and the deduced amino acid sequence had some common domains for UCP, including six mitochondrial carrier protein motifs and a putative purine-nucleotide binding site. RT-PCR and Western blot analyses revealed that both UCP1 mRNA and protein were expressed exclusively in the interscapular adipose tissue. These results suggest that pika UCP1 contributes to heat production in brown adipose tissue, as do those in other species.
解偶联蛋白1（UCP1）仅存在于棕色脂肪组织中，在寒冷暴露期间有助于体温调节。我们克隆了高原鼠兔（Ochotona dauurica）的UCP1 cDNA，高原鼠兔是一种小型、不冬眠、昼行性的兔形目动物，栖息于蒙古和中国北方相对寒冷的气候和高海拔地区。鼠兔UCP1的核苷酸序列与其他物种的UCP1高度同源，推导的氨基酸序列具有一些UCP的共同结构域，包括六个线粒体载体蛋白基序和一个推定的嘌呤核苷酸结合位点。RT-PCR和蛋白质印迹分析表明，UCP1 mRNA和蛋白质仅在肩胛间脂肪组织中表达。这些结果表明，鼠兔UCP1与其他物种的UCP1一样，有助于棕色脂肪组织产热。
