Liang Zhitao, Jiang Zhihong, Ho Hingman, Zhao Zhongzhen
School of Chinese Medicine, Hong Kong Baptist University, Kowloon, Hong Kong Special Administrative Region, P. R. China.
Planta Med. 2007 Nov;73(14):1502-8. doi: 10.1055/s-2007-990259. Epub 2007 Nov 5.
Herba Oldenlandiae is the dried whole herb of Oldenlandia diffusa (Willd.) Roxb. (Family Rubiaceae) recorded in the Chinese pharmacopoeia. However, the herbs of O. corymbosa (L.) Lam and O. tenelliflora Bl. are also often available and used indiscriminately as Herba Oldenlandiae in the markets. In light of this, high-performance liquid chromatography (HPLC) combined with high resolution mass spectrometry (HR-MS) has been developed for a comparative analysis of chemical components in the three Oldenlandia species. The correlation coefficients of the entire chromatographic patterns of samples were calculated by software and used for appraising the similarity. The chemical information on the peaks in the chromatograms of O. diffusa was obtained by comparison of their exact mass data, UV spectra and literature values. The results indicated that the chemical profiling of O. diffusa is quite different in samples collected from different habitats. Furthermore, the variations in the abundance of asperuloside, E-6-O- P-coumaroylscandoside methyl ester and E-6-O- P-coumaroylscandoside methyl ester-10-methyl ether were significant in these related herbs. In conclusion, it is important to use the above three compounds as chemical markers for quality evaluation and chemical authentication of Herba Oldenlandiae and its substitutes.
白花蛇舌草为《中国药典》收载的茜草科植物白花蛇舌草Oldenlandia diffusa (Willd.) Roxb.的干燥全草。然而，伞房花耳草Oldenlandia corymbosa (L.) Lam和纤花耳草Oldenlandia tenelliflora Bl.的全草在市场上也较为常见，且常被不加区分地当作白花蛇舌草使用。鉴于此，已采用高效液相色谱法（HPLC）结合高分辨质谱法（HR-MS）对这三种耳草属植物的化学成分进行比较分析。通过软件计算样品全色谱图的相关系数，并用于评价相似度。通过比较白花蛇舌草色谱图中各峰的精确质量数据、紫外光谱和文献值，获取了其化学信息。结果表明，采自不同产地的白花蛇舌草样品的化学图谱差异较大。此外，在这些相关草药中，车叶草苷、E-6-O-对香豆酰鸡屎藤苷甲酯和E-6-O-对香豆酰鸡屎藤苷甲酯-10-甲醚的含量变化显著。综上所述，将上述三种化合物用作白花蛇舌草及其替代品质量评价和化学鉴别的化学标志物具有重要意义。
