Antonialli W F, Lima S M, Andrade L H C, Súarez Y R
Centro Integrado de Análise e Monitoramento Ambiental, Universidade Estadual de Mato Grosso do Sul (UEMS), Dourados, MS, Brasil.
Genet Mol Res. 2007 Aug 27;6(3):492-9.
Fourier transform-infrared photoacoustic spectroscopy was applied for the first time, to our knowledge, to distinguish different castes of an ant species. The method was applied directly to the abdomen of queens, workers and males of Ectatomma vizottoi ants, without any special sample preparation. The absorption bands of secondary amide and hydrocarbons were identified; using these as variables in a canonical discriminant analysis we found significant differences between the castes. Queens have a greater hydrocarbon content than do workers and males, which is related to their function in the colony. This technique can be used to analyze and distinguish small chemical differences in biological systems, even in opaque samples.
据我们所知，傅里叶变换红外光声光谱法首次被用于区分一种蚂蚁的不同蚁群。该方法直接应用于维佐托伊埃氏蚁蚁后的腹部、工蚁和雄蚁，无需任何特殊的样品制备。识别出了仲酰胺和碳氢化合物的吸收带；在典型判别分析中将这些作为变量，我们发现不同蚁群之间存在显著差异。蚁后的碳氢化合物含量高于工蚁和雄蚁，这与它们在蚁群中的功能有关。该技术可用于分析和区分生物系统中的微小化学差异，即使是不透明的样品。
