利用红外光谱鉴定梨小食心虫（Grapholita molesta (Busk)，鳞翅目：卷蛾科）生物型

Identification of Grapholita molesta (Busk) (Lepidoptera: Tortricidae) Biotypes Using Infrared Spectroscopy.

作者信息

Teixeira R, Fernandéz J I R, Pereira J, Monteiro L B

机构信息

Depto de Entomologia, Univ Federal do Paraná, Curitiba, PR, Brasil,

出版信息

Neotrop Entomol. 2015 Apr;44(2):129-33. doi: 10.1007/s13744-015-0272-y. Epub 2015 Feb 18.

DOI:10.1007/s13744-015-0272-y

PMID:26013130

Abstract

The aim of this study was to determine the differences between two Grapholita molesta (Busk) populations by applying infrared spectroscopy. Grapholita molesta populations were collected from different regions within Porto Amazonas, Brazil, and Emilia Romagna, Italy. Eggs, fifth instars, pupae, and adults were examined. Pupae and adults were sexed. A spectrophotometer that was set between 400 and 4000 cm(-1) range was used to perform 32 scans. The spectra obtained were analyzed using partial least squares (PLS), and external validation of the model was conducted with Unscrambler(TM) software, Version 9.1 (CAMO Software AS, Oslo, Norway). The results showed the differences between the populations in all of the analyzed stages of maturation. The pupal stage was the most viable stage for reading the beam spectrum, and it was concluded that IR can separate G. molesta biotypes.

摘要

本研究的目的是通过应用红外光谱法确定两种梨小食心虫（Grapholita molesta (Busk)）种群之间的差异。梨小食心虫种群采自巴西亚马孙港不同地区以及意大利艾米利亚-罗马涅。对卵、五龄幼虫、蛹和成虫进行了检查。对蛹和成虫进行了性别鉴定。使用一台设置在400至4000 cm(-1)范围内的分光光度计进行32次扫描。所得光谱使用偏最小二乘法（PLS）进行分析，并使用Unscrambler(TM)软件9.1版（挪威奥斯陆CAMO Software AS公司）对模型进行外部验证。结果显示在所有分析的成熟阶段种群之间存在差异。蛹期是读取束光谱最可行的阶段，得出的结论是红外光谱可以区分梨小食心虫生物型。