Jiang Jie, Xue Ru-yi, Zhang Shun-cai, Zhou Jun, Zhou Kang
Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Zhonghua Yi Xue Za Zhi. 2007 Aug 14;87(30):2149-51.
To investigate the effects of intraperitoneal injected schistosome ova on TNBS-induced colitis and on the intestinal TLR4 expression in mice.
40 BALB/c mice were randomized into 3 groups: normal control group (10 mice), TNBS group (20 mice) in which mice were exposed to trinitrobenzesulfonic acid (TNBS) and were induced with colitis, and the schistosome ova group (10 mice) in which mice were intraperitoneal injected with freeze-killed schistosome ova and later exposed to TNBS. The following variables were observed: mortality, pathological appearance of the colon, histological scoring of the specimen, serum TNF-alpha level, and intestinal TLR4 expression detected by RT-PCR and Immunohistochemistry.
Mortality of schistosome ova group was lower than that of the TNBS group (20% vs 70%, P < 0.05). Inflammation of the mice colon in the schistosome ova group was less severe than that of the TNBS group (1.4 +/- 0.5 vs 4.2 +/- 0.6, P < 0.01, Ameho criteria scoring). TLR4 expression of colon was up-regulated in mice of TNBS group and down-regulated in schistosome ova group which was still higher than that of normal controls (0.762 +/- 0.054 vs 0.325 +/- 0.029 vs 0.237 +/- 0.021, P < 0.01).
Intraperitoneal injected schistosome ova can obviously reduce TNBS-induced colitis in mice, which may be attributed to down-regulated TLR4 expression in colon.
研究腹腔注射血吸虫卵对三硝基苯磺酸（TNBS）诱导的小鼠结肠炎及肠道Toll样受体4（TLR4）表达的影响。
将40只BALB/c小鼠随机分为3组：正常对照组（10只小鼠）；TNBS组（20只小鼠），该组小鼠用三硝基苯磺酸诱导结肠炎；血吸虫卵组（10只小鼠），该组小鼠腹腔注射经冷冻杀死的血吸虫卵，随后用三硝基苯磺酸处理。观察以下变量：死亡率、结肠病理外观、标本组织学评分、血清肿瘤坏死因子-α（TNF-α）水平以及通过逆转录聚合酶链反应（RT-PCR）和免疫组织化学检测的肠道TLR4表达。
血吸虫卵组的死亡率低于TNBS组（20%对70%，P<0.05）。血吸虫卵组小鼠结肠的炎症程度低于TNBS组（1.4±0.5对4.2±0.6，P<0.01，阿梅霍标准评分）。TNBS组小鼠结肠的TLR4表达上调，而血吸虫卵组的表达下调，但仍高于正常对照组（0.762±0.054对0.325±0.029对0.237±0.021，P<0.01）。
腹腔注射血吸虫卵可明显减轻TNBS诱导的小鼠结肠炎，这可能归因于结肠中TLR4表达下调。
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