[日本血吸虫卵对三硝基苯磺酸诱导的小鼠结肠炎的抑制作用]

[Suppression of Schistosoma japonicum eggs on TNBS-induced colitis in mice].

作者信息

Mo Hong-mei, Liu Wen-qi, Lei Jia-hui, Cheng Yu-li, Wang Cheng-zu, Li Yong-long

机构信息

Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2007 Oct;25(5):381-4.

PMID:18441989

Abstract

OBJECTIVE

To study the suppression of Schistosoma japonicum eggs against the trinitrobenzene sulfonic acid (TNBS)-induced colitis in mice.

METHODS

50 female BALB/c mice (6-8 week-old) were randomly divided into normal control group, ethanol control group, schistosome egg immunized control group, TNBS-induced colitis group and TNBS-induced colitis with egg immunization group. In TNBS-induced colitis with egg immunization group, mice were immunized 4 times with 10,000 schistosome eggs by intraperitoneal injection with one-week interval. On day 6 after the last immunization the mice were induced by TNBS and the body weight, histological change on colon and level of cytokines of mice were observed in egg-immunized and -unimmunized colitis mice.

RESULTS

The unimmunized mice developed significant inflammation in colon with bloody mucus feces and decreased body weight after TNBS induction. Distinct hyperemia, edema and transmural inflammatory infiltration accompanied with ulceration were shown in colon. The level of IFN-gamma was (3.47 +/- 0.87) ng/ml and IL-4 was (146.06 +/- 45.76) pg/ml. However, in egg-immunized mice, the inflammation was suppressed greatly and the body weight recovered soon after TNBS induction. The production of IL-4 was enhanced to (598.50 +/- 135.90) pg/ml, and IFN-gamma was significantly diminished to (1.53 +/- 0.51) ng/ml.

CONCLUSION

S.japonicum eggs protect mice from colitis induced by TNBS.

摘要

目的

研究日本血吸虫卵对三硝基苯磺酸（TNBS）诱导的小鼠结肠炎的抑制作用。

方法

将50只6 - 8周龄雌性BALB/c小鼠随机分为正常对照组、乙醇对照组、血吸虫卵免疫对照组、TNBS诱导的结肠炎组和TNBS诱导的结肠炎伴虫卵免疫组。在TNBS诱导的结肠炎伴虫卵免疫组中，小鼠腹腔注射10000个血吸虫卵，每周1次，共免疫4次。末次免疫后第6天，用TNBS诱导小鼠，观察虫卵免疫和未免疫的结肠炎小鼠的体重、结肠组织学变化及细胞因子水平。

结果

未免疫小鼠经TNBS诱导后结肠出现明显炎症，有血性黏液便，体重下降。结肠表现为明显充血、水肿、透壁性炎症浸润并伴有溃疡形成。IFN - γ水平为（3.47±0.87）ng/ml，IL - 4水平为（146.06±45.76）pg/ml。然而，在虫卵免疫的小鼠中，炎症得到明显抑制，TNBS诱导后体重很快恢复。IL - 4的产生增加至（598.50±135.90）pg/ml，IFN - γ显著降低至（1.53±0.51）ng/ml。