Reimann Verena, Krämer Ursula, Sugiri Dorothee, Schroeder Peter, Hoffmann Barbara, Medve-Koenigs Kathrin, Jöckel Karl-Heinz, Ranft Ulrich, Krutmann Jean
Institut für umweltmedizinische Forschung, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.
J Invest Dermatol. 2008 May;128(5):1294-7. doi: 10.1038/sj.jid.5701151. Epub 2007 Nov 8.
Sunbeds have become very popular and, as a result, health effects of their use are of growing concern. In this regard, exposure to ultraviolet radiation from sunbeds may cause premature skin aging. Evidence for this assumption, however, has thus far been elusive. In this study, we assessed whether sunbed use induced the generation of a large-scale mitochondrial DNA mutation in human skin, the so-called Common Deletion, which is well known to be photoaging-associated. Fifty-nine individuals who voluntarily started to use sunbeds were observed over 3 months. Punch biopsies were taken from neck and buttock skin before the first and after their last sunbed visit. The Common Deletion content was determined by quantitative real-time PCR. Multiple linear regression analysis was performed for statistical analysis. Volunteers without previous sunbed use showed a 2.56-fold increase (95% CI: 0.97-6.78) of the Common Deletion in neck and a 3.58-fold increase (95% CI: 1.44-8.89) of the Common Deletion in buttock skin samples after sunbed use. Participants with previous sunbed use showed no increase. This study indicates that sunbed use may contribute to mtDNA deletion-associated skin photoaging.
日光浴床已变得非常流行，因此，其使用对健康的影响日益受到关注。在这方面，暴露于日光浴床的紫外线辐射可能会导致皮肤过早老化。然而，这一假设的证据迄今为止一直难以捉摸。在本研究中，我们评估了使用日光浴床是否会导致人类皮肤中大规模线粒体DNA突变的产生，即所谓的常见缺失，众所周知，这种缺失与光老化相关。对59名自愿开始使用日光浴床的个体进行了3个月的观察。在首次使用日光浴床之前和最后一次使用之后，从颈部和臀部皮肤取打孔活检组织。通过定量实时PCR测定常见缺失的含量。进行多元线性回归分析以进行统计分析。以前未使用过日光浴床的志愿者在使用日光浴床后，颈部皮肤样本中常见缺失增加了2.56倍（95%置信区间：0.97 - 6.78），臀部皮肤样本中常见缺失增加了3.58倍（95%置信区间：1.44 - 8.89）。以前使用过日光浴床的参与者则没有增加。这项研究表明，使用日光浴床可能会导致与线粒体DNA缺失相关的皮肤光老化。
