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一株低温烃降解菌的分离鉴定及其降解特性

[Isolation and identification of a low temperature hydrocarbon-degrading strain and its degradation characteristics].

作者信息

Huang Lei, Li Dan, Sun Dan, Xie Yu-juan, Ma Ting, Liang Feng-lai, Liu Ru-lin

机构信息

Tianjin Key Laboratory of Microbial Functional Genomics, College of Life Sciences, Nankai University, Tianjin 300071, China.

出版信息

Huan Jing Ke Xue. 2007 Sep;28(9):2101-5.

Abstract

A low-temperature hydrocarbon-degrading strain T7-2 was isolated from sea-mud of Bohai polluted area and identified as Rhodococcus erythropolis, which could use diesel oil as carbon source. The optimal temperature and pH for the strain utilizing ethanol was 15 degrees C and 7.8, and the optimal concentration of ethanol and the seed culture was 0.5% and 10(8) CFU/mL, respectively. Inoculated to artificial seawater which was added (NH4)2SO5 2.64 g/L, Na2HPO4 2.5 g/L and yeast extract 0.015 g/L after 7 days of culture at the temperature of 15 degrees C, the rate of degradation was 73.2%. The strain could degrade a large range of n-alkane from C12 to C36.

摘要

从渤海污染区海泥中分离出一株低温烃降解菌株T7-2,鉴定为红平红球菌,该菌株能够以柴油为碳源。该菌株利用乙醇的最适温度和pH分别为15℃和7.8,乙醇和种子培养液的最适浓度分别为0.5%和10⁸ CFU/mL。在15℃培养7天后接种到添加了2.64 g/L (NH₄)₂SO₅、2.5 g/L Na₂HPO₄和0.015 g/L酵母提取物的人工海水中,降解率为73.2%。该菌株能够降解从C₁₂到C₃₆的多种正构烷烃。

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