Shukor M Y, Dahalan F A, Jusoh A Z, Muse R, Shamaan N A, Syed M A
Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia 43400 UPM Serdang, Selangor, Malaysia.
J Environ Biol. 2009 Jan;30(1):145-50.
A diesel-degrading bacterium has been isolated from a diesel-polluted site. The isolate was tentatively identified as Staphylococcus aureus strain DRY11 based on partial 16S rDNA molecular phylogeny and Biolog GP microplate panels and Microlog database. Isolate 11 showed an almost linear increase in cellular growth with respect to diesel concentrations with optimum growth occurring at 4% (v/v) diesel concentration. Optimization studies using different nitrogen sources showed that the best nitrogen source was potassium nitrite. Sodium nitrite was optimum at 1.2 g l(-1) and higher concentrations were strongly inhibitory to cellular growth. The optimal pH that supported growth of the bacterium was between 7.5 to 8.0 and the isolate exhibited optimal broad temperature supporting growth on diesel from 27 to 37 degrees C. An almost complete removal of diesel components was seen from the reduction in hydrocarbon peaks observed using Solid Phase Microextraction Gas Chromatography analysis after 5 days of incubation. The characteristics of this bacterium suggest that it is suitable for bioremediation of diesel spills and pollutions in the tropics.
从一个受柴油污染的场地分离出了一种柴油降解细菌。基于部分16S rDNA分子系统发育以及Biolog GP微孔板和Microlog数据库，该分离株初步鉴定为金黄色葡萄球菌DRY11菌株。分离株11的细胞生长随柴油浓度几乎呈线性增加，在柴油浓度为4%（v/v）时生长最佳。使用不同氮源的优化研究表明，最佳氮源是亚硝酸钾。亚硝酸钠在1.2 g l(-1)时最佳，更高浓度对细胞生长有强烈抑制作用。支持该细菌生长的最佳pH值在7.5至8.0之间，该分离株在27至37摄氏度的温度范围内对柴油表现出最佳的广泛温度支持生长。孵育5天后，通过固相微萃取气相色谱分析观察到烃峰减少，柴油成分几乎完全去除。这种细菌的特性表明它适用于热带地区柴油泄漏和污染的生物修复。
