Irian Saeed, Xu Ping, Dai Xinbin, Zhao Patrick X, Roossinck Marilyn J
Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK 73401, USA.
Mol Plant Microbe Interact. 2007 Dec;20(12):1477-88. doi: 10.1094/MPMI-20-12-1477.
Infection of Cucumber mosaic virus (CMV) and D satellite RNA (satRNA) in tomato plants induces rapid plant death, which has caused catastrophic crop losses. We conducted long serial analysis of gene expression (LongSAGE) in control and virus-infected plants to identify the genes that may be involved in the development of this lethal tomato disease. The transcriptomes were compared between mock-inoculated plants and plants infected with CMV, CMV/D satRNA, or CMV/Dm satRNA (a nonnecrogenic mutant of D satRNA with three mutated nucleotides). The analysis revealed both general and specific changes in the tomato transcriptome after infection with these viruses. A massive transcriptional difference of approximately 400 genes was found between the transcriptomes of CMV/D and CMV/Dm satRNA-infected plants. Particularly, the Long-SAGE data indicated the activation of ethylene synthesis and signaling by CMV/D satRNA infection. Results from inoculation tests with an ethylene-insensitive mutant and treatments with an ethylene action inhibitor further confirmed the role of ethylene in mediating the epinastic leaf symptoms and the secondary cell death in the stem. Results from Northern blot analysis demonstrated the partial contribution of ethylene in the induced defense responses in CMV/D satRNA-infected plants.
黄瓜花叶病毒（CMV）和D卫星RNA（satRNA）感染番茄植株会导致植株迅速死亡，这已造成了灾难性的作物损失。我们对对照植株和病毒感染植株进行了基因表达的长链序列分析（LongSAGE），以鉴定可能与这种致死性番茄病害发生发展相关的基因。比较了模拟接种植株与感染CMV、CMV/D satRNA或CMV/Dm satRNA（D satRNA的一个非坏死性突变体，有三个核苷酸发生突变）的植株的转录组。分析揭示了这些病毒感染后番茄转录组的一般变化和特异性变化。在CMV/D和CMV/Dm satRNA感染植株的转录组之间发现了约400个基因的大量转录差异。特别是，Long-SAGE数据表明CMV/D satRNA感染激活了乙烯合成和信号传导。用乙烯不敏感突变体进行接种试验以及用乙烯作用抑制剂进行处理的结果进一步证实了乙烯在介导叶片偏上性症状和茎中次生细胞死亡中的作用。Northern印迹分析结果表明乙烯在CMV/D satRNA感染植株诱导的防御反应中起部分作用。
