Mols Maarten, de Been Mark, Zwietering Marcel H, Moezelaar Roy, Abee Tjakko
Top Institute Food and Nutrition, Wageningen, The Netherlands.
Environ Microbiol. 2007 Dec;9(12):2933-44. doi: 10.1111/j.1462-2920.2007.01404.x.
Bacillus cereus is an important food-borne pathogen and spoilage organism. In this study, numerous phenotypes and the genomes of B. cereus strains ATCC 14579 and ATCC 10987 were analysed to compare their metabolic capacity and stress resistance potential. The growth performance of the two strains was assessed for nearly 2000 phenotypes, including use of nutrient sources, performance in acid and basic environments, osmo-tolerance and antibiotic resistance. Several food-relevant phenotypic differences were found between ATCC 14579 and ATCC 10987, such as differences in utilization of carbohydrates, peptides, amino acids and ammonia. Subsequently, the genomes of both strains were analysed with INPARANOID to search for strain-specific open reading frames (ORFs). B. cereus ATCC 14579 and ATCC 10987 were found to harbour 983 and 1360 strain-specific ORFs respectively. The strain-specific phenotypic features were interlinked with corresponding genetic features and for several phenotypic differences a related strain-specific genetic feature could be identified. In conclusion, the combination of phenotypic data with strain-specific genomic differences has led to detailed insight into the performance of the two B. cereus strains, and may supply indicators for the performance of these bacteria in different environments and ecological niches.
蜡样芽孢杆菌是一种重要的食源性病原体和腐败菌。在本研究中，对蜡样芽孢杆菌菌株ATCC 14579和ATCC 10987的众多表型和基因组进行了分析，以比较它们的代谢能力和抗逆潜力。评估了这两种菌株近2000种表型的生长性能，包括营养源利用、在酸性和碱性环境中的表现、耐渗透压能力和抗生素抗性。在ATCC 14579和ATCC 10987之间发现了几种与食品相关的表型差异，例如碳水化合物、肽、氨基酸和氨利用方面的差异。随后，用INPARANOID分析了两种菌株的基因组，以寻找菌株特异性开放阅读框（ORF）。发现蜡样芽孢杆菌ATCC 14579和ATCC 10987分别含有983个和1360个菌株特异性ORF。菌株特异性表型特征与相应的遗传特征相互关联，对于几种表型差异，可以鉴定出相关的菌株特异性遗传特征。总之，表型数据与菌株特异性基因组差异的结合，使我们对两种蜡样芽孢杆菌菌株的性能有了详细的了解，并可能为这些细菌在不同环境和生态位中的性能提供指标。
