[灵芝遗传多样性的扩增片段长度多态性分析]
[AFLP analysis for genetic diversity of Ganoderma].
作者信息
Zheng Lin-yong, Jia Ding-hong, Luo Xia, Yang Zhi-rong
机构信息
Key Laboratory of Biological Resource and Ecological Environment of the Ministry of Education, College of life Sciences, Sichuan University, Chengdu 610064, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(17):1733-6.
OBJECTIVE
To investigate the genetic diversity of Ganoderma cultivars provided for the genuineness study, germ-plasm resource identification, genetic relationship study, breeding, introduction and cultivante of Ganoderma strains.
METHOD
With the software of NTSYSpc 2. 1, 24 materials, of G. lucidum and G. sinense, were studied using AFLP to construct the dendrogram.
RESULT
There were 177 polymorphic bands with 14 primer combinations. And all materials could be identified with AFLP.
CONCLUSION
There actually existed much genetic diversity at the molecular level among the germplasm resources of Ganoderma strains, and all the strains were clustered into G. lucidum group and G. sinense group at the similarity coefficient 0. 676.
目的
研究用于灵芝品种真伪鉴定、种质资源鉴定、亲缘关系研究、育种、引种及栽培的灵芝菌株的遗传多样性。
方法
利用NTSYSpc 2.1软件，采用扩增片段长度多态性（AFLP）技术对24份灵芝和紫芝材料进行分析，构建聚类图。
结果
14对引物组合共产生177条多态性条带，所有材料均可通过AFLP技术进行鉴别。
结论
灵芝菌株种质资源在分子水平上确实存在丰富的遗传多样性，在相似系数为0.676时，所有菌株聚为灵芝组和紫芝组。
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