Sun Shu-Jing, Gao Wei, Lin Shu-Qian, Zhu Jian, Xie Bao-Gui, Lin Zhi-Bin
College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, People's Republic of China.
Appl Microbiol Biotechnol. 2006 Sep;72(3):537-43. doi: 10.1007/s00253-005-0299-9. Epub 2006 Jan 13.
Genetic marker technology designed to detect naturally occurring polymorphisms at the DNA level had become an invaluable and revolutionizing tool for both applied and basic studies of fungi. To eliminate the confusion on the taxonomy of Ganoderma strains, in this study, a collection of 31 accessions representative of morphotypes and some unclassified types was used for analyzing molecular diversity using a novel molecular marker sequence-related amplified polymorphism (SRAP). This collection included commercial cultivars and wild varieties that represented the great diversification of types from different countries and regions. The experimental results showed that 50 out of 95 combinations of primers turned out to be polymorphic, and 85 polymorphism bands were obtained using six combinations. Based on the appearances of markers, the genetic similarity coefficients were calculated, and genetic variations were observed (0 approximately 1) among the 31 different Ganoderma strains. The group of Ganoderma lucidum showed significant differences from the group of Ganoderma sinense. Moreover, G. lucidum in China was also different from G. lucidum in Yugoslavia. At the same time, cluster analysis successfully categorized these 31 Ganoderma strains into five groups. These results revealed the genetic diversity of Ganoderma strains and their correlation with geographic environments. It also suggested SRAP marker could be used in the taxonomic analysis of fungi. To our knowledge, this is the first application of SRAP marker on the systematics of Ganoderma strains within basidiomycetes.
旨在检测DNA水平上自然发生的多态性的遗传标记技术，已成为真菌应用研究和基础研究中一项非常有价值且具有变革性的工具。为消除灵芝菌株分类学上的混乱，在本研究中，选取了31份代表形态类型及一些未分类类型的材料，利用一种新型分子标记——序列相关扩增多态性（SRAP）分析分子多样性。该材料收集包括商业栽培品种和野生品种，代表了来自不同国家和地区的多种类型。实验结果表明，95对引物组合中有50对具有多态性，使用6对组合获得了85条多态性条带。基于标记的表现计算遗传相似系数，并观察了31种不同灵芝菌株之间的遗传变异（0至1）。灵芝组与紫芝组表现出显著差异。此外，中国的灵芝与南斯拉夫的灵芝也不同。同时，聚类分析成功地将这31种灵芝菌株分为五组。这些结果揭示了灵芝菌株的遗传多样性及其与地理环境的相关性。这也表明SRAP标记可用于真菌的分类分析。据我们所知，这是SRAP标记在担子菌纲灵芝菌株系统学上的首次应用。
