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从海洋细菌弧菌属XY-214菌株中克隆编码β-1,3-木糖苷酶的新基因及其基因产物的特性分析

Cloning of a novel gene encoding beta-1,3-xylosidase from a marine bacterium, Vibrio sp. strain XY-214, and characterization of the gene product.

作者信息

Umemoto Yoshiaki, Onishi Ryosuke, Araki Toshiyoshi

机构信息

Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie 514-8507, Japan.

出版信息

Appl Environ Microbiol. 2008 Jan;74(1):305-8. doi: 10.1128/AEM.01793-07. Epub 2007 Nov 9.

DOI:10.1128/AEM.01793-07
PMID:17993567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2223237/
Abstract

The beta-1,3-xylosidase gene (xloA) of Vibrio sp. strain XY-214 was cloned and expressed in Escherichia coli. The xloA gene consisted of a 1,608-bp nucleotide sequence encoding a protein of 535 amino acids with a predicted molecular weight of 60,835. The recombinant beta-1,3-xylosidase hydrolyzed beta-1,3-xylooligosaccharides to D-xylose as a final product.

摘要

对弧菌属XY-214菌株的β-1,3-木糖苷酶基因（xloA）进行了克隆，并在大肠杆菌中表达。xloA基因由1608个碱基对的核苷酸序列组成，编码一种含有535个氨基酸的蛋白质，预测分子量为60835。重组β-1,3-木糖苷酶将β-1,3-木寡糖水解为最终产物D-木糖。

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本文引用的文献

1
Expression in Escherichia coli and characterization of beta-xylosidases GH39 and GH-43 from Bacillus halodurans C-125.嗜碱芽孢杆菌C-125中β-木糖苷酶GH39和GH-43在大肠杆菌中的表达及特性分析
Appl Microbiol Biotechnol. 2006 Dec;73(3):582-90. doi: 10.1007/s00253-006-0512-5. Epub 2006 Jul 29.
2
The structure of an inverting GH43 beta-xylosidase from Geobacillus stearothermophilus with its substrate reveals the role of the three catalytic residues.来自嗜热栖热放线菌的一种反向GH43β-木糖苷酶与其底物的结构揭示了三个催化残基的作用。
J Mol Biol. 2006 May 26;359(1):97-109. doi: 10.1016/j.jmb.2006.03.005. Epub 2006 Mar 20.
3
Sequencing and expression of the gene encoding the Clostridium stercorarium beta-xylosidase Xyl43B in Escherichia coli.嗜尸梭菌β-木糖苷酶Xyl43B编码基因在大肠杆菌中的测序与表达
Biosci Biotechnol Biochem. 2004 Mar;68(3):609-14. doi: 10.1271/bbb.68.609.
4
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
5
Three acidic residues are at the active site of a beta-propeller architecture in glycoside hydrolase families 32, 43, 62, and 68.在糖苷水解酶家族32、43、62和68中，三个酸性残基位于β-螺旋桨结构的活性位点。
Proteins. 2004 Feb 15;54(3):424-32. doi: 10.1002/prot.10604.
6
Xylan of siphonaceous green algae.管藻目绿藻的木聚糖
Nature. 1960 Jul 2;187:82-3. doi: 10.1038/187082a0.
7
Purification and characterization of beta-1,3-xylanase from a marine bacterium, Alcaligenes sp. XY-234.从海洋细菌产碱杆菌属XY-234中纯化和鉴定β-1,3-木聚糖酶
J Gen Appl Microbiol. 1998 Aug;44(4):269-274. doi: 10.2323/jgam.44.269.
8
Metabolic engineering applications to renewable resource utilization.代谢工程在可再生资源利用中的应用。
Curr Opin Biotechnol. 2000 Apr;11(2):187-98. doi: 10.1016/s0958-1669(00)00085-9.
9
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Appl Environ Microbiol. 2000 Apr;66(4):1741-3. doi: 10.1128/AEM.66.4.1741-1743.2000.
10
Purification, characterization and gene cloning of two alpha-L-arabinofuranosidases from streptomyces chartreusis GS901.来自浅绿链霉菌GS901的两种α-L-阿拉伯呋喃糖苷酶的纯化、表征及基因克隆
Biochem J. 2000 Feb 15;346 Pt 1(Pt 1):9-15.