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来自海洋细菌弧菌属XY - 214菌株的编码β-1,3-木聚糖酶的新基因在大肠杆菌中的克隆、测序及表达

Cloning, sequencing, and expression in Escherichia coli of the new gene encoding beta-1,3-xylanase from a marine bacterium, Vibrio sp. strain XY-214.

作者信息

Araki T, Hashikawa S, Morishita T

机构信息

Faculty of Bioresources, Mie University, Kamihama, Tsu, Mie 514-8507, Japan.

出版信息

Appl Environ Microbiol. 2000 Apr;66(4):1741-3. doi: 10.1128/AEM.66.4.1741-1743.2000.

DOI:10.1128/AEM.66.4.1741-1743.2000
PMID:10742274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC92055/
Abstract

The Vibrio sp. strain XY-214 beta-1,3-xylanase gene cloned in Escherichia coli DH5alpha consisted of an open reading frame of 1, 383 nucleotides encoding a protein of 460 amino acids with a molecular mass of 51,323 Da and had a signal peptide of 22 amino acids. The transformant enzyme hydrolyzed beta-1,3-xylan to produce several xylooligosaccharides.

摘要

克隆于大肠杆菌DH5α中的弧菌属XY-214菌株β-1,3-木聚糖酶基因由1383个核苷酸的开放阅读框组成，编码一个含有460个氨基酸、分子量为51323道尔顿的蛋白质，并且有一个22个氨基酸的信号肽。该转化体酶可水解β-1,3-木聚糖产生多种木寡糖。