通过跨物种互补分析对卡氏肺孢子虫brl1进行功能表征。
Functional characterization of Pneumocystis carinii brl1 by transspecies complementation analysis.
作者信息
Lo Presti Libera, Cockell Moira, Cerutti Lorenzo, Simanis Viesturs, Hauser Philippe M
机构信息
Institute of Microbiology, Centre Hospitalier Universitaire Vaudois and University of Lausanne, 1011 Lausanne, Switzerland.
出版信息
Eukaryot Cell. 2007 Dec;6(12):2448-52. doi: 10.1128/EC.00321-07. Epub 2007 Nov 9.
Abstract
Pneumocystis jirovecii is a fungus which causes severe opportunistic infections in immunocompromised humans. The brl1 gene of P. carinii infecting rats was identified and characterized by using bioinformatics in conjunction with functional complementation in Saccharomyces cerevisiae and Schizosaccharomyces pombe. The ectopic expression of this gene rescues null alleles of essential nuclear membrane proteins of the Brr6/Brl1 family in both yeasts.
摘要
耶氏肺孢子菌是一种可在免疫功能低下的人群中引起严重机会性感染的真菌。利用生物信息学结合酿酒酵母和粟酒裂殖酵母中的功能互补，对感染大鼠的卡氏肺孢子虫的brl1基因进行了鉴定和表征。该基因的异位表达挽救了两种酵母中Brr6/Brl1家族必需核膜蛋白的无效等位基因。
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