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Simultaneous uptake of galactose and glucose by Azotobacter vinelandii.

作者信息

Wong T Y, Murdock C A, Concannon S P, Lockey T D

机构信息

Biology Department, Memphis State University, TN 38152.

出版信息

Biochem Cell Biol. 1991 Oct-Nov;69(10-11):711-4. doi: 10.1139/o91-106.

DOI:10.1139/o91-106
PMID:1799437
Abstract

Azotobacter vinelandii growing on galactosides induced two distinct permeases for glucose and galactose. The apparent Vmax and Km of the galactose permease were 16 nmol galactose/min per 10(10) cells and 0.5 mM, respectively. The apparent Vmax and Km of the glucose permease were 7.8 nmol glucose/min per 10(10) cells and 0.04 mM, respectively. Excess glucose had no effect on the galactose uptake. However, excess galactose inhibited glucose transport. The galactosides-induced glucose permease also exhibited different uptake kinetics from that induced by glucose.

摘要

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