Hovaneissian Michael, Archier Paul, Mathe Carole, Culioli Gérald, Vieillescazes Catherine
Université d'Avignon et des Pays de Vaucluse, Laboratoire de Chimie Bioorganique et des Systèmes Moléculaires Vectoriels, Equipe Chimie appliquée à l'Art et à l'Archéologie, 84000, Avignon, France.
Phytochem Anal. 2008 Jul-Aug;19(4):301-10. doi: 10.1002/pca.1048.
An HPLC and GC study has been conducted on the aromatic oleoresins styrax and benzoin produced by several American, Mediterranean and East-Asian trees, and widely used in ancient civilisations for their therapeutic and odoriferous properties. Initial experiments were performed by HPLC-PAD-fluorimetry for the analysis of several aromatic components, and then completed by GC-MS for the characterisation of both aromatic and triterpenic derivatives. In this work, it was crucial to isolate from fresh natural exudates, and to characterise by two-dimensional NMR, some of the major constituents in order to extend the standard molecular pool prior to chromatographic identifications. This study reveals coniferyl benzoate as an excellent distinctive fluorescent biomarker of Siam benzoin substrate. It also confirms that fluorimetric-coupled detection is a powerful analytical tool for the identification of compounds in Hamamelidaceae extracts that are almost undetectable by UV. GC-MS was successfully applied to the determination of the botanical origin of Sumatra benzoin, and to the identification of lupeol [3beta-lup-20(29)-en-3-ol] for the first time in such balsam-type materials.
对几种美洲、地中海和东亚树木产生的芳香树脂苏合香脂和安息香进行了高效液相色谱（HPLC）和气相色谱（GC）研究，它们因其治疗和芳香特性在古代文明中被广泛使用。最初通过高效液相色谱-光电二极管阵列-荧光分析法对几种芳香成分进行分析，然后通过气相色谱-质谱联用技术对芳香衍生物和三萜衍生物进行表征。在这项工作中，从新鲜天然渗出物中分离并通过二维核磁共振对一些主要成分进行表征至关重要，以便在色谱鉴定之前扩展标准分子库。该研究表明苯甲酸松柏酯是暹罗安息香底物的一种出色的独特荧光生物标志物。它还证实荧光耦合检测是一种强大的分析工具，可用于鉴定金缕梅科提取物中几乎无法通过紫外检测到的化合物。气相色谱-质谱联用技术成功应用于确定苏门答腊安息香的植物来源，并首次在此类香脂型材料中鉴定出羽扇豆醇[3β-羽扇-20(29)-烯-3-醇]。
