Rozenman Mary M, Kanan Matthew W, Liu David R
Howard Hughes Medical Institute and the Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
J Am Chem Soc. 2007 Dec 5;129(48):14933-8. doi: 10.1021/ja074155j. Epub 2007 Nov 10.
We have developed and applied an approach to reaction discovery that takes advantage of DNA encoding, DNA-programmed assembly of substrate pairs, in vitro selection, and PCR amplification, yet does not require reaction conditions that support DNA hybridization. This system allows the simultaneous evaluation of >200 potential bond-forming combinations of substrates in a single experiment and can be applied in a range of solvent and temperature conditions. In an initial application, we applied this system to explore Au(III)-mediated chemistry and uncovered a simple, mild method for the selective Markovnikov-type hydroarylation of vinyl arenes and trisubstituted olefins with indoles.
我们开发并应用了一种反应发现方法,该方法利用了DNA编码、底物对的DNA编程组装、体外筛选和PCR扩增,但不需要支持DNA杂交的反应条件。该系统允许在单个实验中同时评估超过200种潜在的底物成键组合,并且可以应用于一系列溶剂和温度条件。在初步应用中,我们应用该系统探索金(III)介导的化学,并发现了一种简单、温和的方法,用于乙烯基芳烃和三取代烯烃与吲哚的选择性马氏型氢芳基化反应。