利用高峰值功率皮秒半导体激光源产生的超连续谱光进行双光子生物成像。

Two-photon bioimaging utilizing supercontinuum light generated by a high-peak-power picosecond semiconductor laser source.

作者信息

Yokoyama Hiroyuki, Tsubokawa Hiroshi, Guo Hengchang, Shikata Jun-ichi, Sato Ki-ichi, Takashima Keijiro, Kashiwagi Kaori, Saito Naoaki, Taniguchi Hirokazu, Ito Hiromasa

机构信息

Tohoku University, New Industry Creation Hatchery Center (NICHe), 6-6-10 Aramaki-Aoba, Aoba-ku, Sendai 980-8579, Japan.

出版信息

J Biomed Opt. 2007 Sep-Oct;12(5):054019. doi: 10.1117/1.2800393.

DOI:10.1117/1.2800393

PMID:17994907

Abstract

We developed a novel scheme for two-photon fluorescence bioimaging. We generated supercontinuum (SC) light at wavelengths of 600 to 1200 nm with 774-nm light pulses from a compact turn-key semiconductor laser picosecond light pulse source that we developed. The supercontinuum light was sliced at around 1030- and 920-nm wavelengths and was amplified to kW-peak-power level using laboratory-made low-nonlinear-effects optical fiber amplifiers. We successfully demonstrated two-photon fluorescence bioimaging of mouse brain neurons containing green fluorescent protein (GFP).

摘要

我们开发了一种用于双光子荧光生物成像的新方案。我们利用自己开发的紧凑型交钥匙半导体激光皮秒光脉冲源产生的774纳米光脉冲，产生了波长在600至1200纳米的超连续谱（SC）光。超连续谱光在大约1030纳米和920纳米波长处被切片，并使用实验室自制的低非线性效应光纤放大器放大到千瓦峰值功率水平。我们成功地展示了对含有绿色荧光蛋白（GFP）的小鼠脑神经元的双光子荧光生物成像。