Sakadzić Sava, Demirbas Umit, Mempel Thorsten R, Moore Anna, Ruvinskaya Svetlana, Boas David A, Sennaroglu Alphan, Kaertner Franz X, Fujimoto James G
MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Charlestown, Massachusetts 02129, USA.
Opt Express. 2008 Dec 8;16(25):20848-63. doi: 10.1364/oe.16.020848.
Multi-photon microscopy (MPM) is a powerful tool for biomedical imaging, enabling molecular contrast and integrated structural and functional imaging on the cellular and subcellular level. However, the cost and complexity of femtosecond laser sources that are required in MPM are significant hurdles to widespread adoption of this important imaging modality. In this work, we describe femtosecond diode pumped Cr:LiCAF laser technology as a low cost alternative to femtosecond Ti:Sapphire lasers for MPM. Using single mode pump diodes which cost only $150 each, a diode pumped Cr:LiCAF laser generates approximately 70-fs duration, 1.8-nJ pulses at approximately 800 nm wavelengths, with a repetition rate of 100 MHz and average output power of 180 mW. Representative examples of MPM imaging in neuroscience, immunology, endocrinology and cancer research using Cr:LiCAF laser technology are presented. These studies demonstrate the potential of this laser source for use in a broad range of MPM applications.
多光子显微镜（MPM）是生物医学成像的强大工具，能够在细胞和亚细胞水平实现分子对比度以及综合的结构和功能成像。然而，MPM所需的飞秒激光源的成本和复杂性是广泛采用这种重要成像方式的重大障碍。在这项工作中，我们描述了飞秒二极管泵浦Cr:LiCAF激光技术，作为用于MPM的飞秒钛宝石激光器的低成本替代方案。使用每个仅需150美元的单模泵浦二极管，一个二极管泵浦Cr:LiCAF激光器可在约800纳米波长产生持续时间约70飞秒、能量为1.8纳焦的脉冲，重复频率为100兆赫，平均输出功率为180毫瓦。展示了使用Cr:LiCAF激光技术在神经科学、免疫学、内分泌学和癌症研究中进行MPM成像的代表性实例。这些研究证明了这种激光源在广泛的MPM应用中的潜力。
