Schuberth J
National Laboratory of Forensic Chemistry, Toxicology Department, University Hospital, Linköping, Sweden.
Biol Mass Spectrom. 1991 Nov;20(11):699-702. doi: 10.1002/bms.1200201108.
As shown by others, ethanol and methanol appear in the breath of normals, and endogenous methanol becomes detectable also in the blood after intake of ethanol. In this study I have investigated whether low-molecular-weight volatile organics, other than methanol, arise in the blood of drunk drivers who had imbibed alcoholic beverages. To this end a method for searching for such compounds in the blood is described. It was based on headspace extraction, gas chromatographic separation on a DB-WAX capillary, and ion trap detection in the mass range 29-99 u. Detection limits, as defined by the analyte concentration that gives a signal equal to three times the standard deviation of the baseline noise, were estimated for the different mass numbers used in the substance search. Given the detection limits, presented as mmoles per litre (numbers within parentheses), in every drunk driver's blood with more than 10 mmol l-1 of ethanol between seven and nine different volatile substances were spotted. These were ethanol (0.15), 2-propanone (0.015), ethyl acetate (0.0005), 2-butanone (0.006), methanol (1.5), 2-propanol (0.06), ethanol (0.7), 2-butanol (0.03), and 1-propanol (0.03).
正如其他人所表明的，乙醇和甲醇会出现在正常人的呼吸中，并且在摄入乙醇后，血液中也可检测到内源性甲醇。在本研究中，我调查了除甲醇外，饮用酒精饮料的醉酒司机血液中是否会产生低分子量挥发性有机物。为此，描述了一种在血液中寻找此类化合物的方法。该方法基于顶空萃取、DB-WAX毛细管柱气相色谱分离以及在29-99 u质量范围内的离子阱检测。对于物质搜索中使用的不同质量数，估计了检测限，检测限定义为产生等于基线噪声标准偏差三倍信号的分析物浓度。根据以每升毫摩尔数表示的检测限（括号内数字），在每一位血液中乙醇含量超过10 mmol l-1的醉酒司机血液中，发现了7至9种不同的挥发性物质。它们分别是乙醇（0.15）、丙酮（0.015）、乙酸乙酯（0.0005）、丁酮（0.006）、甲醇（1.5）、异丙醇（0.06）、乙醇（0.7）、丁醇（0.03）和丙醇（0.03）。
