Jones A W, Schuberth J
Department of Alcohol Toxicology, National Laboratory of Forensic Chemistry, University Hospital, Linköping, Sweden.
J Forensic Sci. 1989 Sep;34(5):1116-27.
This paper describes the analysis of ethanol in blood specimens from suspect drunk drivers and the associated quality assurance procedures currently used in Sweden for legal purposes. Aliquots of whole blood from two separate Vacutainer tubes are diluted with 1-propanol as internal standard before analysis by headspace gas chromatography (HS-GC) with three different stationary phases: Carbopak B, Carbopak C, and 15% Carbowax 20 M. The actual HS-GC analysis, the integration of chromatographic peaks, the collection and processing of results, as well as the quality control tests involve the use of computer-aided techniques. The standard deviation of analysis(y) increased with concentration of ethanol in the blood specimen(x), and above 0.50 mg/g the regression equation was y = 0.0033 + 0.0153x. The prosecution blood-alcohol concentration (BAC) is the mean of three separate determinations made by different laboratory technicians working independently with different sets of equipment. A deduction is made from the mean analytical result to compensate for random and systematic errors inherent in the method. At BACs of 0.5 and 1.5 mg/g, which are the statutory limits in Sweden, the allowances currently made are 0.06 and 0.09 mg/g, respectively. Accordingly, the reduced prosecution BAC is less than the actual BAC with a statistical confidence of 99.9%.
本文描述了对涉嫌酒后驾车者血液样本中乙醇的分析，以及瑞典目前用于法律目的的相关质量保证程序。在通过顶空气相色谱法（HS-GC）使用三种不同固定相（Carbopak B、Carbopak C和15% Carbowax 20 M）进行分析之前，将来自两个单独真空采血管的全血等分试样用1-丙醇作为内标进行稀释。实际的HS-GC分析、色谱峰积分、结果的收集和处理以及质量控制测试都涉及计算机辅助技术的使用。分析的标准偏差（y）随着血液样本中乙醇浓度（x）的增加而增加，在浓度高于0.50 mg/g时，回归方程为y = 0.0033 + 0.0153x。检方的血液酒精浓度（BAC）是由不同实验室技术人员使用不同设备独立进行的三次单独测定的平均值。从平均分析结果中扣除一部分以补偿该方法固有的随机和系统误差。在瑞典法定限值的0.5和1.5 mg/g的BAC水平下，目前的扣除量分别为0.06和0.09 mg/g。因此，可以以99.9%的统计置信度确定，检方降低后的BAC低于实际BAC。
